Liver injuries and alterations of gut microbiota in intrahepatic cholestasis in pregnancy of rats
10.19405/j.cnki.issn1000-1492.2023.05.020
- Author:
Liuyuan He
1
;
Bin Zhang
1
;
Heping Zhang
1
;
Jilong Shen
2
;
Yuanhong Xu
2
;
Xuhang Shen
3
Author Information
1. Dept of Laboratory Diagnostics, Maternal and Child Medical Center of Anhuui (Anhui Medical University) ,Hefei 230001
2. Dept of Laboratory Diagnostics,The First Hospital of Anhui Medical Uniiversity, Key Laboratory of PathogeniBiology of Anhui Province,Hefei 230022
3. Dept of Gastroenterology,The First Hospital of Anhui Medical University, Hefei 230022
- Publication Type:Journal Article
- Keywords:
intrahepatic cholestasis in pregnancy;
rat model;
gut microbiota;
16S rDNA
- From:
Acta Universitatis Medicinalis Anhui
2023;58(5):824-829
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of hepatobiliary metabolie dysfunction on gut microbiota in rats with intrahepatic cholestasis of pregnancy (ICP).
Methods:Forty Sprauge-Dawley(SD) rats at 10 days of gestation were randomly divided into two groups,25 for ICP induction and 15 as control. Estradiol benzoate, combined with progesterone, was given to the rats by intraperitoneal injjection from 10 to 14 days after gestation. Hepatic impairment indicators of total bilirubin(TBIL), alanine aminotransferase (ALT), aspartate aminotransferase (AST),-glutamyl
transferase ( GGT), alkaline phosphatase(AL.P), and total bile acids (TBA) in sera of the animals were detected in each group to verify the model construction. Simultaneously, the liver tissues were subjected to pathology examination and fresh faeces samples were collected for 16S rDNA sequencing to explore the gut microbiota profiles.
Results:The serum levels of TBIL,ALT,AST,GGT,ALP and TBA in ICP group were significantly elevated compared with the control (P<0. 05 );the ICP rats presented obvious characteristics of hepatobiliary disorders, showing prominent steatosis, necrosis of hepatocytes and infiltration of inflammatory cells, with formation of bile thrombus in some animals. The abundance of Eubacterium, Bacteroides, Parabacteroides distasonis and Bacteroides uniformis remarkably decreased wereas Prevotela and Fusobacterium significantly increased in ICP rats when compared with the control (P<0.05).
Conclusion:The hepatic injuries and bile acid metabolic disorders occurred in ICP rats,which resulted in dysbiosis of gut microbiota.
- Full text:2024081222283267610妊娠期肝内胆汁淤积症大鼠肝损伤及其肠道菌群结构的研究_何刘媛.pdf
