Ginsenoside Rg1 promotes proliferation , migration and osteogenic differentiation of human gingival fibroblasts
10.19405/j.cnki.issn1000-1492.2023.05.018
- Author:
Xin Zhang
1
,
2
;
Changshun Li
2
;
Hao Liu
2
;
Shaoyue Zhu
2
;
Meng Zhou
2
;
Yan Feng
2
;
Guangdong Zhang
1
Author Information
1. Dept of Oral and Maxillofacial Surgery, the Afiliated Stomatological Hospital of Nanjing Medical University, Jiangsu Province Key Laboratory of Oral Diseases ,Jiangsu Province Engineering Researching Center of Stomatological Translation Medicine ,Nanjing 210029
2. Dept of Oral and Maxillofacial Surgery, the Afiliated Stomatological Hospital of Xuzhou Medical University,Xuzhou 221002
- Publication Type:Journal Article
- Keywords:
human gingival fibroblasts;
ginsenoside Rg1;
cell proliferation;
cell migration;
osteogenesis differentia⁃ tion
- From:
Acta Universitatis Medicinalis Anhui
2023;58(5):812-819
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Ginsenoside Rg1 (GsRg1) on proliferation , migration and osteogenic differentiation of human gingival fibroblasts (HGFs) and its molecular mechanism.
Methods:Human gingival fibroblasts (HGFs) were isolated and cultured by tissue block method , and identified by morphology and immunofluorescence. The effect of six concentrations of GsRg1 (0 , 6. 25 , 12. 5 , 25 , 50 , 100 mg/L) on the proliferation of HGFs was detected by CCK⁃8 method. Transwell assay was used to detect the effects of different concentrations of GsRg1 on the migration ability of HGFs. Alkaline phosphatase (ALP) staining was used to detect osteogenic ability. Alizarin red staining was used to observe and quantify calcium nodules. The expression of COL⁃ Ⅰ , OCN and OPN osteogenic genes was detected by qRT⁃PCR. Western blot was used to detect the protein expression of OCN ,OPN , COL⁃ Ⅰ and PI3K/AKT signaling pathway.
Results:Compared with the control group , the proliferation ability of HGFs was significantly improved at the concentrations of 12. 5 ,25 ,50 and 100 mg/L GsRg1 (P < 0. 05) , and the proliferation promotion effect of 100 mg/L GsRg1 was the strongest. There was no significant difference between the 6. 25 mg/L GsRg1 group and the control group. After GsRg1 treatment , the migration ability of HGFs was enhanced and showed concentration dependence. Compared with the control group , the activity of ALP in 100 mg/L GsRg1 group significantly increased (P < 0. 01) . Alizarin red staining showed a significant increase in the number of calcium nodules(P < 0. 01) . The mRNA and egg white expression levels of osteogenic genes OCN , OPN and COL⁃ Ⅰ increased (P < 0. 05 ) . The expression levels of p ⁃PI3K and p ⁃Akt were significantly up⁃regulated with time ( P <0. 05) ,while the expression levels of PI3K and AKT had no significant changes.
Conclusion :GsRg1 can promote the proliferation and migration of HGFs , and 100 mg/L GsRg1 can promote the osteogenic differentiation of HGFs ,which may be related to the activation of PI3K/AKT signaling pathway.
- Full text:2024081221562350512人参皂苷Rg1促进人牙龈成...增殖、迁移及成骨分化的研究_张昕.pdf
