Comprehensive evaluation of Yifei qinghua ointment by multi-component quantitative analysis combined with chemometrics and entropy weight TOPSIS
- VernacularTitle:多成分定量分析结合化学计量学和熵权TOPSIS法综合评价益肺清化膏质量
- Author:
Xiongfei WAN
1
;
Meifeng LIANG
2
;
Nian LIAO
1
;
Shanshan ZHU
1
;
Zhijian WANG
3
Author Information
1. Dept. of Pharmacy，China Resources & WISCO General Hospital Affiliated to Wuhan University of Science and Technology，Wuhan 430080，China
2. Dept. of Medical Insurance，China Resources & WISCO General Hospital Affiliated to Wuhan University of Science and Technology，Wuhan 430080，China
3. School of Pharmacy，Hubei University of Chinese Medicine，Wuhan 430070，China
- Publication Type:Journal Article
- Keywords:
Yifei qinghua ointment;
HPLC method;
multi-
- From:
China Pharmacy
2024;35(15):1837-1843
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To comprehensively evaluate the quality of Yifei qinghua ointment by multi-component quantitative analysis combined with chemometrics and entropy weight-technique for order preference by similarity to ideal solution （TOPSIS） method. METHODS The contents of lobetyolin， syringin， calycosin 7-O-β-D-glucopyranoside， ononin， astraisoflavan-7-O-β-D- glucoside， isomucronulatol 7-O-glucoside， astragaloside Ⅳ ， deapi-platycoside E， platycoside E， platycodin D3， feretoside， asperulosidic acid， asperuloside， methylophiopogonanone A and methylophiopogonanone B in 14 batches of Yifei qinghua ointment （S1-S14） were determined by high-performance liquid chromatography method. Then， the quality of 14 batches of Yifei qinghua ointment was analyzed by chemometrics （principal component analysis and orthogonal partial least-squares discriminant analysis） and entropy weight TOPSIS method. RESULTS The results of chemometrics showed that 14 batches of Yifei qinghua ointment could be clustered into three categories， S1-S6 as the first category， S7-S10 as the second category， and S11-S14 as the third category. The values of variable importance for projection of calycosin 7-O-β-D-glucopyranoside， ononin， feretoside， astragaloside Ⅳ， astraisoflavan-7-O-β-D-glucoside， lobetyolin， methylophiopogonanone A and platycoside E were higher than 1. The results of the entropy weight TOPSIS method showed that the Euclidean closeness of the optimal solution of 14 batches of Yifei qinghua ointment were between 0.152 9 and 0.736 6， and that of sample S14 was the highest （0.736 6）. CONCLUSIONS Among 14 batches of Yifei qinghua ointment， sample S14 has the best quality， and 8 components such as calycosin 7-O-β-D-glucopyranoside and ononin may be differential markers affecting the quality of Yifei qinghua ointment.
