The mechanism of long non⁃coding RNA SNHG16 regulating the expression of miR⁃195⁃5p to promote the proliferation and migration of gastric cancer cells
10.19405/j.cnki.issn1000-1492.2023.09.021
- Author:
Juan Kang
1
;
Jiao He
2
;
Weihong Ren
2
Author Information
1. Henan University of Chinese Medicine , Zhengzhou 450046
2. The First Afiliated Hospital of Henan University of Chinese Medicine , Zhengzhou 450000
- Publication Type:Journal Article
- Keywords:
lncRNA SNHG16;
miR⁃195 ⁃5p/MYB;
gastric cancer cell;
proliferation;
migration
- From:
Acta Universitatis Medicinalis Anhui
2023;58(9):1564-1571,1579
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect of lncRNA SNHG16 (SNHG16) on the proliferation and migration
of gastric cancer cells and its molecular regulatory mechanism.
Methods :The expression of SNHG16 in gastric cancer tissue was retrieved based on online database and the downstream target gene of SNHG16 was screened. The interaction between SNHG16 and miR⁃195 ⁃5p was verified by bioinformatics analysis and double luciferase reporter gene experiment. The expression of SNHG16 , miR⁃195 ⁃5p and MYB in gastric cancer cells (HGC⁃27 , MKN⁃28) was detected by real⁃time fluorescent quantitative PCR (qRT⁃PCR) ; SNHG16 was knocked down to detect the expression of miR⁃195 ⁃5p or MYB ; The expression of MYB was detected by overexpression of miR⁃195 ⁃5p;Western blot analysis was used to detect the protein expression level of MYB in each group. Knock down SNHG16 or overexpress miR⁃195 ⁃5p , and the proliferation and migration ability of gastric cancer cells (HGC⁃27 , MKN⁃28) were detected through cell proliferation and scratch test.
Results :The expression of LncRNA SNHG16 increased in gastricancer tissues and gastric cancer cells (HGC⁃27 , MKN⁃28 , MKN⁃45 , NCI⁃N87) . The results of double luciferase reporter gene experiment showed that psiCHECK2⁃SNHG16⁃WT and miR⁃195⁃5p mimic were transfected into HGC⁃27 at the same time , significantly inhibiting the luciferase activity of HGC⁃27 cells. The results of qRT PCR and WB experiments showed that knocking down SNHG16 in HGC⁃27 and MKN⁃28 cells upregulated miR⁃195⁃5p and inhibited the expression of MYB at the transcription and translation levels ; Overexpression of miR⁃195 ⁃5p in HGC⁃27 and MKN⁃28 cells inhibited MYB expression. The results of CCK8 proliferation test and cell scratch test showed that knocking down SNHG16 or overexpressing miR⁃195 ⁃5p could inhibit the proliferation and migration of HGC⁃27 and MKN⁃28 cells.
Conclusion : LncRNA SNHG16 can regulate the expression of MYB in gastric cancer cells through miR⁃195 ⁃5p , and the high expression of SNHG16 can promote the proliferation and migration of gastric cancer cells.
- Full text:202408121642055719LncRNA_SNHG16...YB促进胃癌细胞增殖及迁移_康娟.pdf
