Expression of TRIM32 in cutaneous malignant melanoma and its correlation with prognosis
10.19405/j.cnki.issn1000-1492.2023.09.019
- Author:
Danyang Zhao
1
;
Dongsheng Cao
1
;
Jiegou Xu
2
;
Juan Xie
1
Author Information
1. Dept of Plastic Surgery, The Second Afiliated Hospital of Anhui Medical University, Hefei 230601
2. Dept ofImmunology,School of Basic Medicine , Anhui Medical University, Hefei 230032
- Publication Type:Journal Article
- Keywords:
TRIM32;
cutaneous melanoma;
immunohistochemistry;
prognosis
- From:
Acta Universitatis Medicinalis Anhui
2023;58(9):1551-1556
- CountryChina
- Language:Chinese
-
Abstract:
Objectives : To investigate the expression and clinical significance of TRIM32 in Cutaneous Malignant Melanoma patients.
Methods :Real⁃time PCR was used to detect the expression of TRIM32 in human melanoma cell lines (A375 , MV3) and human normal skin cell lines (HACAT) . Clinical characteristics of 31 CMM patients were collected. The expression of TRIM32 in the CMM tissues and para⁃cancerous tissues was detected by immunohistochemistry (IHC) . The correlation between TRIM32 expression and clinical characteristics was analyzed. Kaplan⁃Meier survival analysis and Cox univariate analysis model were used to analyze the prognostic factors.
Results:The expression of TRIM32 mRNA in A375 melanoma cells was significantly higher than that in HACAT normal skin cells (P < 0. 001) ,while the expression in MV3 cells was comparable to that in HACAT cells. The expression of TRIM32 protein in the CMM tissues , detected by IHC , was significantly higher than that in the para⁃cancerous tissues (P < 0. 001) and positively correlated with Breslow thickness( r = 0. 771 , P < 0. 001) and Clark grade ( r =0. 635 ,P < 0. 001) , but not with sex , ulcer, age and primary location of tumor ( P > 0. 05) . Also , TRIM32 expression level was significantly correlated with prognosis of CMM.
Conclusion :TRIM32 is highly expressed in A375 melanoma cells and CMM tissues. TRIM32 may be helpful for the prognosis of CMM patients.
- Full text:2024081216314621492TRIM32在皮肤黑色素瘤中的表达及预后相关性分析_赵丹阳.pdf
