Screening of host RNA binding proteins interacting with Nelson Bay reovirus σ NS by immunoprecipitation combined with mass spectrometry
10.19405/j.cnki.issn1000-1492.2023.09.018
- Author:
Runlin Li
1
;
Siman Hu
1
;
Like Luo
1
;
Xuejiao Jia
1
;
Mengqi Liu
1
;
Yonggang Li
1
;
Xiaoli Tao
1
Author Information
1. Laboratory of Pathogenic Biology,School of Basic Medical Sciences , Jinzhou Medical University,Jinzhou 121000
- Publication Type:Journal Article
- Keywords:
nelson nay orthoreoviruses;
RNA binding proteins;
σNS protein;
interaction protein;
immunoprecipitation
- From:
Acta Universitatis Medicinalis Anhui
2023;58(9):1546-1550
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To screen the RNA binding proteins interacting with NBV nonstructural protein σNS in host
cells and to analyze their bioinformatics functions.
Methods :In this study , the eukaryotic expression vector pEF⁃HA⁃MB⁃S3 of NBV σ NS was constructed and transfected into HEK293T cells after verification. After RNase A treatment , the obtained protein lysate was enriched by immunoprecipitation to enrich σNS binding proteins , identified and analyzed by LC⁃MS/MS mass spectrometry , and the properties and specific functions of proteins were discovered with the help of related Bioinformatics tools.
Results :In this study , 32 candidate RNA binding proteins interacting with NBV σNS proteins were successfully screened , and the results of bioanalysis showed that these proteins were mainly located in cytoplasm and nucleus , and were mainly involved in biological processes such as cell metabolism , biological regulation , virus translation and transcription.
Conclusion :This study preliminarily analyzes the function of RNA binding proteins interacting with NBV σNS , which lays a foundation for further study on the mechanism of σNS protein in NBV life cycle.
- Full text:2024081216282992568免疫沉淀联合质谱筛选与纳尔...S互作的宿主RNA结合蛋白_李润林.pdf
