Expression and significance of eIF2B1 in hepatocellular carcinoma
10.19405/j.cnki.issn1000-1492.2023.05.016
- Author:
Wenyue Qi
1
;
Dachen Zhou
2
;
Zhenhua Zhang
1
;
Guomei Xia
1
;
Wei Chen
2
;
Guizhou Zou
1
Author Information
1. Dept ofHepatology,the Second Afiliated Hospital of Anhui Medical University,Hefei 230601
2. Dept ofHepatobiliary Surgery, the Second Afiliated Hospital of Anhui Medical University,Hefei 230601
- Publication Type:Journal Article
- Keywords:
hepatocellular carcinoma;
HBV;
eIF2B1;
eIF2α;
protein translation;
prognosis
- From:
Acta Universitatis Medicinalis Anhui
2023;58(5):799-805
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of eukaryotic translation initiation factor 2B1(eIF2B1) in HCC and its correlation with clinical prognosis.
Methods:Through the follow⁃up of the clinical data of 743 hepatocellular carcinoma patients in the specimen bank ,91 HBV⁃related liver cancer patients with complete follow⁃up information were screened out , and their postoperative tumor tissues were selected. Meanwhile , the adjacent tumor tissues with a
distance of more than 2 cm from the tumor margin were collected to make tissue chips. Western blot and immunohistochemical experiments were performed. Image J was used to analyze the proportion of positive cells stained by tissue chip and the gray value of Western blot results. Clinical data and follow⁃up data of included patients were statistically analyzed by R4. 0. 5 software.
Results:Immunohistochemical results suggested that eIF2B1 was more strongly expressed in HCC than in para⁃cancerous tissues. Western blot results showed that eIF2B1 was more strongly expressed in HepG2. 2. 15 cells than in HepG2 cells. The expression of eIF2B1 in HCC tissues was correlated with
tumor number (P < 0. 05) . Cox multivariate regression analysis showed that the expression of eIF2B1 was an independent risk factor for the overall clinical prognosis of HBV⁃DNA positive patients (HR = 4. 28 ,P = 0. 018) .
Conclusion:The expression of eIF2B1 is significantly enhanced in HBV⁃associated HCC tissues and is significantly
associated with overall survival in HBV DNA positive patients.
- Full text:2024081211022003626eIF2B1在肝细胞癌中的表达及意义_齐文月.pdf
