Porphyromonas gingivalis infection of esophageal cancer cells induces M2 macrophage polarization and promotes esophageal cancer progression
10.19405/j.cnki.issn1000-1492.2023.05.013
- Author:
Jingyi Guo
1
,
2
;
Xiang Yuan
3
,
4
;
Linlin Shi
3
,
4
;
Xiusen Zhang
4
;
Jinyu Kong
3
,
4
;
Dingyu Zhang
1
,
2
;
Shegan Gao
3
,
4
Author Information
1. Basic Medical College of Henan University of Science and Technology,Luoyang 471003
2. Henan Key Laboratory of Microbiome and Esophageal Cancer Prevention and Treatment ,Henan Key Laboratory of Cancer Epigenetics ,Luoyang 471003
3. Henan Key Laboratory of Microbiome and Esophageal Cancer Prevention and Treatment ,Henan Key Laboratory of Cancer Epigenetics ,Luoyang 471003
4. The First Afiliated Hospital of Henan University of Science and Technology, Clinical Medicine College of Henan University of Science and Technology,Luoyang 471003
- Publication Type:Journal Article
- Keywords:
esophageal squamous cell carcinoma;
Porphyromonas gingivalis;
cytokines;
THP⁃1 cells;
macrophages
- From:
Acta Universitatis Medicinalis Anhui
2023;58(5):780-787
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Porphyromonas gingivallis ( Pg) infection of esophageal cancer cells on the polarization of tumor associated macrophages (TAMs) and functional changes.
Methods:The secretion of tumor⁃related cytokines in the supernatant of Pg infected and uninfected esophageal squamous cell carcinoma (ESCC) cells was detected by ELISA. A co⁃culture model of ESCC cells and macrophages in vitro was established ,and the changes of TAMs surface markers were detected by qPCR , cellular immunofluorescence and flow cytometry. Cytokines secreted by TAMs after co⁃culture were detected by ELISA. ESCC cells were cultured using conditioned medium of co⁃cultured TAMs , and the effects of TAMs on the proliferation , migration and invasion of esophageal cancer cells were evaluated by CCK⁃8 , Wound⁃healing assay and Transwell assay.
Results:The expression quantity of IL⁃6 and IL⁃10 of Pg⁃infected ESCC cells increased (P < 0. 01) . The contents of CD163 and CD206 on the surface of TAMs co⁃cultured with Pg⁃infected ESCC cells increased (P < 0. 001) . The cytokines IL⁃6 and IL⁃10 secreted by TAMs co⁃cultured with Pg⁃infected ESCC cells relatively increased (P < 0. 01) . TAMs co⁃cultured with Pginfected ESCC cells were able to enhance ESCC cells proliferation , migration and invasion (all P < 0. 05) .
Conclusion:Pg infection of ESCC cells can induce the secretion of cytokines , remodel TAMs to polarize toward the M2type immunosuppressive phenotype , thereby promoting the malignant biological behavior of ESCC cells. This study provides data support for the etiology of esophageal cancer and potential target molecules for clinical immunotherapy targeting TAMs.
- Full text:2024081210423848136牙龈卟啉单胞菌感染食管癌细...巨噬细胞极化促进食管癌进展_郭静宜.pdf
