A preliminary study of dihydroartemisinin inhibiting migration invasion and vasculogenic mimicry of non⁃small cell lung cancer cells
10.19405/j.cnki.issn1000-1492.2023.05.011
- Author:
Bingqi Hu
1
;
Jing Zhou
1
;
Junfeng Huang
1
;
Liwen Chen
1
Author Information
1. Dept ofLaboratory Medicine , the Second Hospital of Anhui Medical University, Hefei 230601
- Publication Type:Journal Article
- Keywords:
dihydroartemisinin;
non⁃small cell lung cancer cells;
epithelial⁃mesenchymal transition;
vasculogenic mimicry
- From:
Acta Universitatis Medicinalis Anhui
2023;58(5):766-771
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effects of dihydroartemisinin ( DHA) on cell growth , migration invasion
and vasculogenic mimicry ability of non⁃small cell lung cancer ( NSCLC) .
Methods :Different concentrations of DHA were added to NSCLC cell lines A549 and H3255 , and after 24 hours , the cell viability was detected by CCK8 method , and the migration and invasion ability of NSCLC cells was detected by Transwell experiment . qRT⁃PCR and Western blot detected E ⁃cadherin , N ⁃cadherin , and Vimentin mRNA and protein expression levels , respectively .Three⁃dimensional cell was cultured to observe the vascular⁃like morphological generation of cells . qRT⁃PCR and Western blot detected human vascular endothelial cadherin (VE⁃cadherin) mRNA and protein expression levels as marker of vasculogenic mimicry .
Results :DHA inhibited cell growth of A549 and H3255 and showed time⁃dependent and concentration⁃dependent . DHA inhibits the invasion and metastasis ( all P < 0. 001) of A549 and H325 cells ; DHA upregulated the expression of E ⁃Cadherin at mRNA(all P < 0. 001) and protein(P < 0. 001;P < 0. 01) levels in A549 and H3255 cells , and downregulated the expression of N ⁃cadherin at mRNA (all P < 0. 01) and protein (all P < 0. 001) levels as well as the expression of Vimentin at mRNA (P < 0. 01;P < 0. 001) and protein (all P < 0. 001) levels . The results of three⁃dimensional cell culture showed that the 12⁃hour vasculogenic mimicry generation capacity of DHA⁃treated A549 cells and H3255 cells was reduced , and the expressions of VE⁃cadherin at mRNA (P < 0. 001;P < 0. 01) and protein (all P < 0. 001) levels were downregulated .
Conclusion :Dihydroartemisinin inhibits the growth , migration invasion and vasculogenic mimicry ability of non⁃small cell lung cancer cells
- Full text:2024081210273103420双氢青蒿素抑制非小细胞肺癌...袭和血管生成拟态的初步研究_胡冰琪.pdf
