Research on Wnt2/β⁃catenin pathway changes in liver regeneration and repair in C57BL/6 mice
10.19405/j.cnki.issn1000-1492.2023.05.009
- Author:
Luyuan Yao
1
,
2
;
Yun Liu
3
;
Qian Yang
1
,
2
;
Xin Bao
3
;
Yan Wang
3
;
Xiaoying Zhao
3
;
Junming Tang
3
Author Information
1. Hubei Key Laboratory ofEmbryonic Stem Cell Research , Faculty of Basic Medical Sciences , Hubei University of Medicine , Shiyan 442000
2. Dept ofAnesthesiology, Taihe Hospital , Hubei University of Medicine , Shiyan 442000
3. Hubei Key Laboratory ofEmbryonic Stem Cell Research , Faculty of Basic Medical Sciences , Hubei University of Medicine , Shiyan 442000
- Publication Type:Journal Article
- Keywords:
partial hepatectomy;
liver regeneration and repair;
Wnt2;
β ⁃catenin
- From:
Acta Universitatis Medicinalis Anhui
2023;58(5):753-759
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the changing characteristics of Wnt2/β⁃catenin pathway in liver regeneration and repair in C57BL/6 mice.
Methods :Male C57BL/6 mice were randomly divided into a sham⁃operated group (Sham group) , 1 d post⁃operative group , 2 d post⁃operative group , 4 d post⁃operative group , 6 d post⁃operative group , and 8 d post⁃operative group. Mice in the operated group underwent partial hepatectomy ( PHx) to remove the left and middle lobes of the liver, respectively. Plasma and liver tissues were collected on postoperative days 1 , 2 ,4 ,6 , and 8 , and plasma ALT and AST activity was measured by ALT(alanine aminotransferase) and AST( aspartate aminotransferase) biochemical analysis kits; Ki67 ⁃positive cells were identified by immunohistochemistry in each group;the number of HNF4⁃α and Ki67 double positive cells , the number of LYVE1 and Ki67 double positive cells , and the number of β ⁃catenin transferred into the nucleus were determined by immunofluorescence method. The expression of Wnt2 protein in each group was detected by Western blot , and the time characteristics of its expression during liver regeneration were analyzed.
Results:Liver weight and liver/body weight ratio peaked on day 6 after PHx and approached the level of the Sham group. After PHx , there was severe liver damage on day 1 , but it had normalized by day 4. On day 2 after PHx , mainly hepatocytes proliferated;and on days 4 and 6 , mainly liver sinusoidal endothelial cells proliferated ,while the Wnt2/β⁃catenin pathway was activated.
Conclusion:The liver has a powerful regenerative repair ability ,which is closely related to the rapid proliferation of hepatocytes and liver sinusoidal endothelial cells , and the activation of the Wnt2/β⁃catenin pathway is activated in the regenerative repair of the mouse liver.
- Full text:202408121012565534Wnt2_β-cateni..._6小鼠肝再生修复中的变化_姚路远.pdf
