Sulforaphane down⁃regulates the expression of eIF4F translation initiation complex in colorectal cancer
10.19405/j.cnki.issn1000-1492.2022.10.024
- Author:
Yun Xing
1
;
Dachen Zhou
1
;
Liang He
1
;
Xiao Cui
1
;
Zongfan Yu
1
Author Information
1. Dept of General Surgery, The Second Affiliated Hospital of Anhui Medical University, Hefei 230601
- Publication Type:Journal Article
- Keywords:
colorectal cancer;
sulforaphane;
eIF4F complex;
HT⁃29;
4EBP1;
immunohistochemistry
- From:
Acta Universitatis Medicinalis Anhui
2022;57(10):1645-1650
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To study the relationship between the expression of eIF4F complex and human colorectal cancer (CRC), and the possible mechanism of sulforaphane on the proliferation of CRC cells.
Methods :Immunohistochemical staining was used to detect the expression of eIF4F complex related proteins in tumor and adjacent tissues of 12 colorectal cancer patients. MTT colorimetry was used to detect the cell activity of colorectal cancer cells treated with sulforaphane. Western blot was used to detect the expression of eIF4F complex related proteins in colorectal cancer cells treated with sulforaphane. In the animal experiment ( tumor formation in nude mice), the growth of subcutaneous tumors in nude mice after intraperitoneal injection of sulforaphane was compared, and the expressions of PI3K/AKT/mTOR/4EBP1 signal pathway related proteins and eIF4F complex related proteins were detected by immunohistochemical staining.
Results :In the specimens of CRC patients, the expression of eIF4F complex related protein in tumor tissues was significantly higher than that in paracancerous tissues. SFN could inhibit the proliferation of CRC cells, and the higher the concentration, the stronger the inhibitory ability. SFN could inhibit the growth and development of CRC cells and down⁃regulate the expression of eIF4F complex in CRC cells.
Conclusion :The expression of eIF4F complex is closely related to the development of colorectal cancer. Sulforaphane may affect the up⁃regulation of eIF4F complex through PI3K/AKT/mTOR/4EBP1 signal pathway, thus affecting the development of colorectal cancer cells.
- Full text:2024081121585145572萝卜硫素下调结直肠癌中eIF4F翻译起始复合物的表达_邢运.pdf
