Effect of CAR⁃NK⁃92 targeting MSLN on ovarian cancer
10.19405/j.cnki.issn1000-1492.2022.10.021
- Author:
Yao Ge
1
,
2
;
Qi Liu
2
;
Chunyan Wang
2
;
Shupeng Liu
2
;
Zhongping Cheng
1
,
2
Author Information
1. Anhui Medical University, Shanghai Clinical College , Shanghai 200072
2. Dept of Obstetrics and Gynecology, Shanghai Tenth Peoples Hospital , Tongji University, Shanghai 200072
- Publication Type:Journal Article
- Keywords:
natural killer cells;
chimeric antigen receptor;
mesothelin;
ovarian cancer
- From:
Acta Universitatis Medicinalis Anhui
2022;57(10):1627-1632
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the killing effect of chimeric antigen receptor NK⁃92 (CAR⁃NK⁃92) cells targeting mesothelin (MSLN) on ovarian cancer cells.
Methods :The expression of MSLN in primary ovarian cancer tissues and ovarian cancer cell lines was detected by immunohistochemical analysis and immunofluorescence, respectively. The CAR⁃NK⁃92 cells targeting MSLN were constructed by lentiviral transfection, the transfection efficiency was detected by flow cytometry, and the killing effect of CAR⁃NK⁃92 on ovarian cancer was verified in vivo and in vitro.
Results :MSLN was highly expressed in primary ovarian cancer tissues and ovarian cancer cell lines. Flow
cytometry showed that the purity of CAR⁃NK⁃92 targeting MSLN was about 70% . The experiments found that MSLN⁃CAR⁃NK⁃92 cells had a strong anti⁃ovarian cancer effect in vivo and in vitro , and could release more cytokines interferon⁃γ and tumor necrosis factor⁃α .
Conclusion :MSLN⁃CAR⁃NK⁃92 has strong anti⁃ovarian cancer effects in vivo and in vitro, providing a new potential treatment option for ovarian cancer patients.
- Full text:2024081121420483218靶向间皮素的CAR-NK-92对卵巢癌的作用研究_葛瑶.pdf
