Effects of hypoxic preconditioning on energy metabolism of mitochondria in mouse hippocampal HT22 cells
10.19405/j.cnki.issn1000-1492.2022.10.014
- Author:
Ruifang Qi
1
,
2
;
Na Li
1
,
2
;
Lijun Wang
1
;
Jun Lv
1
;
Ruili Shi
1
;
Baohui Ma
1
;
Jinghua Shi
1
;
Xiaoqiong Hao
1
;
Guo Shao
3
Author Information
1. Dept of Physiology, College of Basic Medicine and Forensic Medicine , Baotou Medical College of Inner Mongolia University of Science and Technology,Baotou 014040
2. Key Laboratory of Hypoxic Translational Medicine of Inner Mongolia , Baotou 014040
3. Translational Medicine Center of The Third Peoples Hospital of Longgang District , Shenzhen City of Guangdong Province , Shenzhen 518172
- Publication Type:Journal Article
- Keywords:
hypoxic preconditioning;
mitochondrial metabolism;
mTOR;
autophagy;
neuroprotection
- From:
Acta Universitatis Medicinalis Anhui
2022;57(10):1585-1588,1594
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect of hypoxic preconditioning (HPC) on mitochondrial energy metabolism in mouse hippocampal HT22 cells and its possible mechanism.
Methods :In this paper, mouse hippocampal nerve cells HT22 were divided into control group, hypoxia group, HPC group, and the levels of adenosine triphosphate (ATP) and reactive oxygen species (ROS) in each group were measured for observing the effect of HPC on cell mitochondrial metabolism. Western blot was used to detect the expression of target of rapamycin ( mTOR), phosphorylated mTOR protein and autophagy substrate P62 protein; cellular immunofluorescence was used to detect phosphorylated mTOR, and LysoTrackerTM probe was used to detect lysosomes.
Results :Compared with the control group, the ATP level was significantly decreased and the ROS level was increased in the hypoxia group. Exposed to HPC, the ATP level was increased and the ROS level was decreased. Compared with the control group, the expression of phosphorylated mTOR was down⁃regulated and the expression of autophagy substrate P62 was down⁃regulated in the HPC group.
Conclusion :HPC may affect the energy metabolism of HT22 cells through the mTOR/autophagy signaling pathway, thereby exerting a protective effect on the HT22 cells.
- Full text:2024081120385361170低氧预适应对小鼠海马HT22细胞线粒体能量代谢的影响_齐瑞芳.pdf
