Repulsive effects of FGF3 on the inhibitory GABAergic axons in the prethalamus
10.19405/j.cnki.issn1000-1492.2022.10.012
- Author:
Hong Huang
1
;
Shuyang Yu
1
;
Weiyun Zhang
1
;
Li Xiao
1
;
Zhengang Xu
1
;
Fang Liu
1
Author Information
1. Dept of Cell Biology, School of Basic Medical Sciences , Nanchang University, Nanchang 330031
- Publication Type:Journal Article
- Keywords:
FGF3;
GABA neurons;
axon guidance
- From:
Acta Universitatis Medicinalis Anhui
2022;57(10):1574-1578
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To study the guidance effects of fibroblast growth factor 3 ( FGF3) on the prethalamic γ⁃aminobutyric acid (GABA) inhibitory axons, and to explore the effect of FGF3 on the formation of thalamic axonal
network.
Methods :Expression and localization of FGF3 and prethalamic GABA marker gene in chicken embryonic
diencephalon were detected by immunofluorescence. Early orientation of GABA axons was tracked by DiI. Three⁃dimensional gel co⁃culture experiment was carried out to investigate guidance effects of FGF3 on early prethalamic GABA inhibitory axons.
Results : Prethalamic GABA cells were adjacent to the FGF3 + hypothalamas, located above the hypothalamus. DiI tracing experiments revealed that prethalamic GABA inhibitory axons had already extended into the thalamus at E6. Compared with the blank control group, FGF3 not only significantly promoted the growth of prethalamic GABA inhibitory nerve fibers in the prethalamus, but also repelled the newborn prethalamic axons to the dorsal thalamus. The number of prethalamic axons was significantly less in proximal section (towards FGF3 beads) than that in distal section (away from FGF3 beads) (P < 0. 01) . Moreover, the guidance effects of FGF3 on prethalamic axons could be blocked by the FGF pathway inhibitor SU5402.
Conclusion :FGF3, an axon guidance molecule expressed in hypothalamus, exerts guidance effects on the pathway selection of adjacent prethalamic GABA inhibitory nerve fibers.
- Full text:2024081120220419944纤维母细胞生长因子3对前丘...丁酸能抑制性轴突的排斥作用_黄红.pdf
