Effect of mandelic acid on the proliferation , apoptosis and migration ability of lung adenocarcinoma H1299 cells and its mechanism
10.19405/j.cnki.issn1000-1492.2023.05.007
- Author:
Haiqing Lu
1
;
Yanli Li
2
;
Zihan Hua
1
;
Jiaxin Xiao
2
;
Bo Ling
1
;
Guangbin Ye
2
Author Information
1. School ofPharmacy,YouJiang Medical University for Nationalities ,Baise 533000
2. School of Basic Medical Sciences , YouJiang Medical University for Nationalities ,Baise 533000
- Publication Type:Journal Article
- Keywords:
lung adenocarcinoma;
mandelic acid;
proliferation;
apoptosis;
migration
- From:
Acta Universitatis Medicinalis Anhui
2023;58(5):742-747
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The research was aimed at exploring the effects of mandelic acid on the proliferation , apoptosis and migration of H1299 lung adenocarcinoma (LUAD) cells and the related molecular mechanisms.
Methods:CCK⁃8 was used to detect changes in the proliferation ability of H1299 cells;Hoechst 33258/PI double⁃staining assay was used to analyze the apoptosis of H1299 cells;Transwell and migration assays were used to analyze changes in the invasive and migration ability of H1299 cells;Western blot was used to detect the expression of proliferation ,apoptosis and migration⁃related pathway proteins.
Results :Different concentrations of mandelic acid inhibited the proliferation viability and invasion and migration ability of H1299 cells (P < 0. 05) . Mandelic acid induced high expression of bax and cl⁃caspase⁃3 and low expression of p ⁃stat3 in proliferation and apoptosis pathways (P < 0. 05) .In addition , it inhibited the expression of MMP⁃9 and Vimentin proteins ,which were related to invasion and migration (P < 0. 01) .
Conclusion:Mandelic acid inhibited the proliferation of H1299 LUAD cells and elevated the level of apoptosis , and molecular biological mechanism might be closely related to the decreased activation of stat3 and activation of bax/caspase⁃3 signaling axis. The inhibition of invasive and migrational ability of H1299 cells induced by mandelic acid was associated with the decreased expression of MMP⁃9 and Vimentin proteins.
- Full text:2024081023573328243扁桃酸对肺腺癌H1299细...亡和迁移能力的影响及其机制_陆海青.pdf
