Crisaborole ointment regulates abnormal activation of keratinocytes and relieves psoriasis in mice
10.19405/j.cnki.issn1000-1492.2023.05.006
- Author:
Yuqing Gui
1
;
Caihong Tang
1
;
Jingyu Chen
1
;
Ling Jiang
2
,
3
;
Wei Wei
1
Author Information
1. Institute of Clinical Pharmacology of Anhui Medical University, Hefei 230032
2. Institute of Clinical Pharmacology of Anhui Medical University, Hefei 230032
3. The First Afiliated Hospital of University of Science and Technology of China , Hefei 230001
- Publication Type:Journal Article
- Keywords:
psoriasis;
crisaborole;
proliferation;
differentiation;
keratinocytes;
PKA;
CREB
- From:
Acta Universitatis Medicinalis Anhui
2023;58(5):735-741
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To study the effect of crisaborole on imiquimod (IMQ) Ⅳinduced psoriasis in mice.
Methods:Forty eight Balb/c mice were randomly divided into crisaborole group (7. 5 , 15 , 30 mg/cm2），halometasone group ( 15 mg/cm2 ) , model group and normal group. IMQ was applied to the back of mice to establish the psoriasis model. Psoriasis area and severity index ( PASI) score was calculated , pathological changes , skin epidermal thickness and inflammatory cell infiltration in the dermis were observed by HE staining. The expressions of keratin (K) 1 , K10 , K6 , K16 and K17 in skin lesions were detected by Western blot and immunohistochemistry. The levels of cyclic adenosine monophosphate ( cAMP) , protein kinase A ( PKA) and phospho⁃cAMP response element binding protein (p⁃CREB) were detected.
Results :Compared with the model group , the PASI score of the crisaborole group decreased , the expression levels of proliferative keratin ( K6 , K16 and K17 ) decreased( F = 12. 62、19. 41、28. 39 ,P < 0. 01) , and the expression levels of differentiation keratin (K1 and K10) increased(F = 27. 95、9. 64 , P < 0. 01) .
Conclusion:Crisaborole plays a therapeutic role in IMQ⁃induced psoriasis in mice by regulating the abnormal proliferation and differentiation of keratinocytes.
- Full text:2024081022502289688克立硼罗软膏调节角质形成细...异常活化缓解小鼠银屑病作用_桂雨晴.pdf
