Role of serum exosomal miR⁃30d⁃5p targeting RHOB in occupational medicamentosa⁃like dermatitis due to trichloroethylene
10.19405/j.cnki.issn1000-1492.2023.05.003
- Author:
Shuyang Cai
1
;
Hui Wang
2
,
3
;
Xuesong Zhang
1
;
Xulei Zuo
1
;
Jinru Ma
1
;
Yiting Hong
1
;
Qifeng Wu
4
;
Qixing Zhu
2
,
3
,
5
Author Information
1. Dept of Occupational Hygiene and Environmental Hygiene ,School of Public Health , Anhui Medical University,Hefei 230032
2. Dept ofDermatology, the First Afiliated Hospital of Anhui Medical University,Hefei 230022
3. Key Laboratory ofDermatology,Ministry of Education , the First Afiliated Hospital of Anhui Medical University,Hefei 230022
4. Guangdong Province Hospital for Occupational Disease Prevention and Treatment , Guangzhou 510000
5. Dept of Occupational Hygiene and Environmental Hygiene ,School of Public Health , Anhui Medical University,Hefei 230032
- Publication Type:Journal Article
- Keywords:
trichloroethylene;
exosomes;
miR⁃30d⁃5p;
RHOB
- From:
Acta Universitatis Medicinalis Anhui
2023;58(5):718-723,730
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detect the expression of serum exosomal miR⁃30d⁃5p in occupational dermatitis medicamentosa⁃like of trichloroethylene(OMDT) patients and its correlation with liver function , then perform bioinformatics analysis and verify the target gene.
Methods :Serum exosomes were extracted from 6 OMDT patients and 6 healthy controls , and miRNA was extracted from exosomes which were identified by transmission electron microscopy, nanoparticle tracking analysis and western blotting. The expression of serum exosomal miR⁃30d⁃5p was detected by real⁃time fluorescence quantitative PCR , then the correlation with liver function was analyzed. The target genes of miR⁃30d⁃5p were predicted by the miRWalk and miRBD databases. Gene ontology analysis and KEGG pathways analysis were performed. Finally , RHOB was verified by Dual⁃luciferase reporter assay.
Results:The expression of serum exosomal miR⁃30d⁃5p significantly decreased in 6 OMDT patients at the peak of the disease(P < 0. 05) , and it was negatively correlated with the level of AST , ALT and GGT (correlation coefficient : rs = - 0. 943 ,P = 0. 005 ;rs = - 0. 886 , P = 0. 019 ; rs = - 0. 886 , P = 0. 019 ) . Bioinformatics analysis and dual⁃luciferase reporter assay showed that RHOB was the target gene of miR⁃30d⁃5p.
Conclusion:The expression of serum exosomal miR⁃30d-5p decreases in OMDT patients , which is negatively correlated with the level of liver function , and the target gene RHOB may be involved in the process of liver injury induced by trichloroethylene.
- Full text:2024081022190710619血清外泌体miR-30d-...三氯乙烯药疹样皮炎中的作用_蔡曙阳.pdf
