Determination of astragaloside Ⅳ in Jianer Xiaoshi oral liquid by HPLC-CAD
10.19778/j.chp.2024.01.017
- VernacularTitle:HPLC-CAD法测定健儿消食口服液中黄芪甲苷的含量
- Author:
ZHAO Chunhe
- Publication Type:Journal Article
- Keywords:
Jianer Xiaoshi oral liquid;
astragaloside;
HPLC-CAD;
content determination;
verify;
improvement
- From:
Drug Standards of China
2024;25(1):099-0103
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an HPLC-CAD method to determine the content of astragaloside in Jianer Xiaoshi oral liquid in order to improve the content determination method in the current standard.
Methods: Agilent ZORBAX SB-C18 column (4.6 mm×250 mm,5 μm) was adopted with acetonitrile-water (35:65) as the mobile phaseat the flow rate of 1 mL·min-1 by gradient elution.Column temperature was room temperature, and theoretical plate number was ≥4 000. Detection parameters of electrospray detector: control mode remote (N2000,1:local0), atomizing tube temperature 25 ℃, carrier gas (nitrogen) was at flow rate: 4 L·min-1. The feasibility and reliability of this method for the determination of astragaloside were confirmed by various methods.
Results: The method showed a good linear relationship in the concentration range of 2.25-101.68 μg·mL-1. RSD of content was 1.35% in repeatability test with average content of 0.824 mg·mL-1. The average recovery rate （n=3） was 99.2% with RSD 1.2%, which met the requirements.
Conclusion: The method verified by methodology can be applied quality control of Jianer Xiaoshi oral liquid method.
- Full text:202408101325275035420240217.pdf
