Simultaneous determination of four active ingredients including gentiopicrin in Jindan tablets by HPLC

VernacularTitle:HPLC法同时测定金胆片中龙胆苦苷等4种主成分的含量
Author: WU Yong ; LIU Yan ; DING Ru ; YU Lu ; XU Tingting
Publication Type:Journal Article
Keywords: HPLC; Jindan tablets; gentiopicrin; polydatin; quercetin; emodin; content
From: Drug Standards of China 2024;25(1):094-098
CountryChina
Language:Chinese
Abstract: Objective: To establish a method to determine 4 active ingredients in Jindan tablets by HPLC.
Methods: Agilent Eclipse X DB C₁₈ (4.6 mm×250 mm, 5 μm) column was adoped using acetonitrile (phase A) -0.1% phophoric acid solution (phase B) as the mobile phase with gradient program at the flow rate of 1.0 mL·min^-1, the detection wavelength was 270 nm, Column temperature was set at 30 ℃.
Results: The linear ranges of gentiopicrin, polydatin, quercetin and emodin were 7.875-78.75 μg·mL^-1（r=0.999 9）, 6.75-67.50 μg·mL^-1（r=0.999 7）, 7.726-77.26 μg·mL^-1（r=0.999 4）, 3.809-38.09 μg·mL^-1（r=0.999 8), respectively, the peak area had a good linear relationship with the mass concentration of 4 components, the average recovery were 99.31%, 99.21%, 99.04%, 99.59%, (n=6) respectively, and RSDs were 1.86%, 1.24%, 1.37%, 1.15%, respectively.
Conclusion: This method is reliable, repeatable and stable, and has strong specificity. This method can provide a reference for the quality control and standard improvement of Jindan tablets.
Full text:20240810132514057520240216.pdf