Simultaneous determination of calceolarioside B and chlorogenic acid in Caulis Stauntoniae Chinensis tablets by HPLC
10.19778/j.chp.2024.01.015
- VernacularTitle:HPLC法同时测定野木瓜片中木通苯乙醇苷B和绿原酸的含量
- Author:
HE Jifen
;
HUANG Guojian
;
FU Dianhai
;
XIE Jiong
;
LÜ Guanxin
- Publication Type:Journal Article
- Keywords:
Caulis Stauntoniae Chinensis tablets;
HPLC;
calceolarioside B;
chlorogenic acid;
content determination
- From:
Drug Standards of China
2024;25(1):090-094
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for simultaneous determination of calceolarioside B and chlorogenic acid in Caulis Stauntoniae Chinensis tablets by HPLC.
Methods: The analysis was performed on a Thermo BDS HYPERSIL C18 column (4.6 mm×250 mm,5 μm) maintained at 35 ℃. The mobile phase was consisted of methanol and 0.1% phosphoric acid solution, and gradient eluted with a flow rate of 1.0 mL·min-1. The detection wavelength was 327 nm, and the injection volume was 10 μL.
Results: The linear ranges of calceolarioside B and chlorogenic acid were 0.51-20.60 μg·mL-1 (r=1.000) and 0.52-20.63 μg·mL-1 (r=1.000), respectively. The average recoveries were 100.3% with RSD as 1.1% and 105.9% with RSD as 1.4%, respectively. The content results of 5 batches of Caulis Stauntoniae Chinensis tablets were 0.083-1.115 mg·g-1 for calceolarioside B and 0.161-1.204 mg·g-1 for chlorogenic acid.
Conclusion: The method can be used for improving the quality evaluation standard of Caulis Stauntoniae Chinensis tablets.
- Full text:20240810132458223720240215.pdf
