The clinical application of targeted next-generation sequencing and metagenomics next-generation sequencing in pathogenic microorganism detection.
10.3760/cma.j.cn112150-20230201-00072
- Author:
Cong Lin ZHAO
1
;
Kai LIU
1
;
Yong Zhao ZHOU
2
Author Information
1. Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu 610041, China.
2. Department of Respiratory and Critical Care Medicine, West China Hospital, Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Humans;
Anti-Bacterial Agents/therapeutic use*;
High-Throughput Nucleotide Sequencing/methods*;
Metagenomics/methods*;
Communicable Diseases
- From:
Chinese Journal of Preventive Medicine
2024;58(1):114-121
- CountryChina
- Language:Chinese
-
Abstract:
Infectious diseases seriously threaten human health. The early detection of the causative organism and antibiotic resistance genes of infection remains a major clinical challenge. Next-generation sequencing has been widely used in diagnosing infectious diseases. Cheaper and faster targeted next-generation sequencing technology is progressively being used in clinical practice. However, the awareness among clinicians about the clinical utility of this technology is limited. The article presents the theoretical background, advantages and disadvantages, clinical applications of targeted next-generation sequencing, and its contrasts with metagenomics next-generation sequencing. It provides a reference for clinicians to select laboratory methods for identifying pathogens in clinical practice.
