Analysis of gene characteristics and core genome characteristics of carbapenem-resistant Enterobacter cloacae in rural residents of Weifang City, Shandong Province.
10.3760/cma.j.cn112150-20230412-00287
- Author:
An Ge WANG
1
;
Shuang WANG
2
;
Lu LIU
2
;
Gao Xiang SUN
2
;
Yu Zhen CHEN
2
;
Jia Xin QI
1
;
Zeng Qiang KOU
2
;
Zhen Wang BI
1
Author Information
1. Hospital Infection Management Office, Provincial Hospital Affiliated to Shandong First Medical University (Shandong Academy of Medical Sciences), Jinan 250021, China.
2. Infectious Disease Control Center, Shandong Center for Disease Control and Prevention, Jinan 250014, China.
- Publication Type:Journal Article
- MeSH:
Humans;
Anti-Bacterial Agents/therapeutic use*;
Enterobacter cloacae/genetics*;
Multilocus Sequence Typing;
beta-Lactamases/genetics*;
Phylogeny;
Rural Population;
Carbapenems/pharmacology*;
Microbial Sensitivity Tests
- From:
Chinese Journal of Preventive Medicine
2024;58(1):48-55
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the drug-resistant gene characteristics and core genome characteristics of carbapenem-resistant Enterobacter cloacae (CR-ECL) in rural residents of Weifang City, Shandong Province. Methods: Fecal samples were collected from rural community residents in Weifang City, Shandong Province in 2017. Drug-resistant strains were screened using a carbapenem-resistant enterobacter chromogenic medium. CR-ECL positive strains were acquired via Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry(MALDI-TOFMS) analysis. The antibiotic resistance phenotype of CR-ECL was determined using a microbroth dilution assay. Whole genome sequencing (WGS) and analysis were conducted, along with an examination of the immediate vicinity of the blaNDM gene and phylogenetic analysis of the strains. Results: A total of 628 fecal samples were collected and tested, of which 6 were CR-ECL positive (detection rate 0.96%), all exhibiting multiple drug resistance (MDR) phenotypes. Six CR-ECL strains had four MLST genotypes (ST), all of which carried multiple drug resistance genes (blaNDM-1, blaNDM-5, etc.) and virulence genes (acrA, acrB, entB, fepC, etc.). There were mobile genetic elements ISAba125, TN3-IS3000, TN3 and IS5 in the genetic environment surrounding the blaNDM gene. The phylogenetic tree showed that the multi-locus sequence typing of the core genome (cgMLST) was consistent with the single nucleotide polymorphism (SNPs) results. The cgMLST results showed that the allele differences between strains 2BC0101B and 2BC0251B, 2BG0561B and 2BI0221B were 2 and 1, respectively. The SNPs results showed that the above two pairs of bacteria also clustered together. It was found that the strains of chicken fecal samples in the National Center for Biotechnology Information (NCBI) database were located in the center of the evolutionary tree, and the local sequences could be traced back to American human sequences. Conclusion: Multidrug-resistant CR-ECL is detected in rural community residents in Weifang City, Shandong Province.
