Analysis of hemoglobin variants in Tianjin City and neighboring areas.
10.3760/cma.j.cn112150-20230408-00272
- Author:
Yu LIU
1
;
Rui LIU
1
;
Yi Song DAI
2
;
Xiao Yan GUO
3
;
Wen Yan NIU
4
Author Information
1. Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Chu Hsien-I Memorial Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin 300134,China.
2. National Health Commisssion Key Laboratory of Pulmonary Immue-related Diseases, Guizhou Provincial People's Hospital, Guiyang 550000,China.
3. Department of Pathology, Jinchang People's Hospital of Hexi Branch, Jinchang 737202,China.
4. Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Chu Hsien-I Memorial Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University, Tianjin 300134,China Department of Immunology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070,China.
- Publication Type:Journal Article
- MeSH:
Humans;
Glycated Hemoglobin;
Case-Control Studies;
Retrospective Studies;
Hospitals
- From:
Chinese Journal of Preventive Medicine
2023;57(12):2147-2152
- CountryChina
- Language:Chinese
-
Abstract:
To determine the types and proportion of common hemoglobin variants in Tianjin and surrounding areas, to analyze the recognition ability and the effects of hemoglobin variants on experimental results in two commonly used glycated hemoglobin systems, so as to provide data support for the consistency of HbA1c detection in Tianjin City. A case-control study was used for retrospective analysis,156 specimens with abnormal electrophoretic peaks in the detection of glycated hemoglobin were collected from more than 50 000 specimens of patients in Chu Hsien-I Memorial Hospital of Tianjin Medical University between June 2020 and December 2020. Determined their hemoglobin mutation sites by DNA sequencing, and compared the values of hemoglobin variants on glycated hemoglobin detection values by high performance liquid chromatography and capillary electrophoresis. SPSS 23 was used to calculate the blood routine results of the variant specimens, and compared with the normal reference interval. The results showed that DNA sequencing identified 21 hemoglobin variants, of which 11 were α strand variants and 10 were β strand variants. In addition, an unreported hemoglobin variant was identified, Hb Headington (HBB: c.217A>C). The HbA1c of 11 variants including Hb G-Honolulu, Hb Queens, Hb Q-Thailand, Hb J-Broussais, Hb O-Indonesia, Hb G-Coushatta, Hb G-Taipei, Hb E, Hb Headington, Hb New York and Hb D-Los Angeles were shifted by more than 7% when measured by high-performance liquid chromatography. Patients with the Hb Q-Thailand and Hb E cause reduced MCV and MCH. In conclusion, an unreported hemoglobin variant was found from Tianjin and neighboring areas. Patients with the Hb Q-Thailand and Hb E cause reduced MCV and MCH. 11 of these hemoglobin variants interfered with the detection of glycated hemoglobin using high-performance liquid chromatography, resulting in inaccurate results.
