Effects of Ondansetron and Granisetron on Rocuronium-induced Neuromuscular Blockade in a Rat Phrenic Nerve-hemidiaphragm Preparation.
10.4097/kjae.2006.51.1.94
- Author:
Chong Wha BAEK
1
;
Hyun KANG
;
Yong Hun JUNG
;
Jung Won PARK
;
Hwa Sung JUNG
;
Yoon Kyung LEE
;
Hong Seuk YANG
Author Information
1. Department of Anesthesiology and Pain Medicine, College of Medicine, Chung-Ang University, Seoul, Korea.
- Publication Type:In Vitro ; Original Article
- Keywords:
drug interaction;
granisetron;
neuromuscular blockade;
ondansetron;
rocuronium
- MeSH:
Animals;
Antiemetics;
Drug Interactions;
Granisetron*;
Ligand-Gated Ion Channels;
Logistic Models;
Neuromuscular Blockade*;
Ondansetron*;
Rats*;
Receptors, Nicotinic;
Serotonin
- From:Korean Journal of Anesthesiology
2006;51(1):94-100
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Both ondansetron and granisetron exert their antiemetic effects via a blockade of the 5-hydroxytryptamine 3 receptor (5-HT(3)R). Because the 5-HT(3)R is a member of a superfamily of ligand-gated ion channels and has structural similarities to the nicotinic acetylcholine receptor (nAChR), a 5-HT(3)R antagonist may also inhibit the nAChR. This study examined the effects of 5-HT(3)R antagonists, ondansetron and granisetron, on rocuronium-induced neuromuscular blockade in vitro. METHODS: Rat phrenic nerve-hemidiaphragm preparations were isolated and allocated randomly into seven groups (control, 1, 10, 100 microgram/ml of ondansetron, 0.1, 1, 10 microgram/ml of granisetron). Two studies were carried out using single twitch responses. In the cumulative dose-response study, rocuronium 1 microgram/ml and each doses of ondansetron or granisetron were administered simultaneously, and incremental 0.5 microgram/ml doses of rocuronium were added to obtain more than 95% neuromuscular twitch inhibition. ED(5), ED(50), ED(90), and ED(95) of rocuronium in each group were calculated using a logistic model. In the partial curarization study, the twitch heights were measured after administering ondansetron or granisetron (10 minutes after administering 3 microgram/ml rocuronium) and were measured 10 minutes later. The 2 twitch heights were then compared. RESULTS: In the cumulative dose-response study, ondansetron 100 microgram/ml and granisetron 10 microgram/ml significantly reduced the ED50 of rocuronium (P < 0.05). There were no intergroup differences in the partial curarization study. CONCLUSIONS: High concentration of ondansetron and granisetron enhanced the neuromuscular blockade of rocuronium. Granisetron enhanced the neuromuscular blockade of rocuronium more potently than ondansetron.
