The clinical application of targeted next-generation sequencing and metagenomics next-generation sequencing in pathogenic microorganism detection
10.3760/cma.j.cn112150-20230201-00072
- VernacularTitle:靶向二代测序技术与宏基因组二代测序技术在病原微生物检测中的应用
- Author:
Conglin ZHAO
1
;
Kai LIU
;
Yongzhao ZHOU
Author Information
1. 四川大学华西医院感染性疾病中心，成都 610041
- Keywords:
Metagenomics;
Sequence analysis;
Infection;
Microbiota;
Drug resistance
- From:
Chinese Journal of Preventive Medicine
2024;58(1):114-121
- CountryChina
- Language:Chinese
-
Abstract:
Infectious diseases seriously threaten human health. The early detection of the causative organism and antibiotic resistance genes of infection remains a major clinical challenge. Next-generation sequencing has been widely used in diagnosing infectious diseases. Cheaper and faster targeted next-generation sequencing technology is progressively being used in clinical practice. However, the awareness among clinicians about the clinical utility of this technology is limited. The article presents the theoretical background, advantages and disadvantages, clinical applications of targeted next-generation sequencing, and its contrasts with metagenomics next-generation sequencing. It provides a reference for clinicians to select laboratory methods for identifying pathogens in clinical practice.
