Antimicrobial resistance and genomic characterization of Campylobacter isolates recovered from retailed poultry meat samples in 20 provinces of China in 2020.
10.3760/cma.j.cn112150-20230222-00145
- Author:
Chang Wei WANG
1
;
Yao BAI
2
;
Shao Ting LI
3
;
Zi Xin PENG
4
;
Da Jin YANG
4
;
Yin Ping DONG
2
;
Jing XIAO
1
;
Wei WANG
1
;
Feng Qin LI
2
Author Information
1. Division IV of Food Safety Standards, China National Center for Food Safety Risk Assessment, Beijing 100021, China.
2. National Health Commission Key Laboratory of Food Safety Risk Assessment of Health, China National Center for Food Safety Risk Assessment, Beijing 100021, China.
3. School of Biomedical and Phamaceutical Sicences, Guangdong University of Technology, Guangzhou 510006, China.
4. National Center for Food Safety Risk Assessment, Beijing 100021, China.
- Publication Type:Journal Article
- MeSH:
Humans;
Animals;
Anti-Bacterial Agents/pharmacology*;
Campylobacter/genetics*;
Poultry;
Drug Resistance, Bacterial/genetics*;
Genomics;
China;
Tetracycline
- From:
Chinese Journal of Preventive Medicine
2023;57(12):2086-2094
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To understand the antimicrobial resistance and genome characteristics of Campylobacter isolates recovered from retailed poultry meat samples in 20 provinces in China in 2020. Methods: In 2020, 265 Campylobacter strains including 244 Campylobacter jejuni and 21 Campylobacter coli collected from retailed poultry meat samples in China were tested for antimicrobial resistance to 9 antimicrobial compounds by using the agar dilution method. Forty-two selected isolates were sent for whole genome sequencing and 38 high-quality genomes were analyzed for their antimicrobial resistance genes, virulence genes, sequence types and genetic diversity. Results: The resistance rates of Campylobacter isolates from poultry meats to tetracycline, nalidixic acid and ciprofloxacin were the highest (84%-100%), with 53.2% of the isolates showing multidrug resistance in this study. The resistance rates of C. coli to erythromycin, azithromycin, telithromycin, gentamicin and clindamycin were significantly higher than those of C. jejuni (P<0.05). The resistance genes conferring resistance to β-lactams (100%, 38/38), quinolones (94.7%, 36/38), tetracycline (81.6%, 31/38) and aminoglycosides (50%, 19/38) were the most frequently detected among 38 Campylobacter genomes. C. jejuni carried more virulence genes than C. coli. In total, 19 and 17 sequence types (ST) were obtained from 20 sequenced C. jejuni and 18 C. coli isolates, respectively, including 5 novel STs. The isolates showed a high genetic diversity based on their sequence types. Conclusion: The phenomenon of antimicrobial resistance in Campylobacter from poultry meat sources in China is relatively serious, and resistance and virulence genes are widely distributed in Campylobacter. There is genetic diversity in Campylobacter.
