Extent of Mycobacterium bovis infection in dairy cattle herds subject to partial culling as determined by an interferon-gamma assay.
10.4142/jvs.2014.15.2.259
- Author:
Sungmo JE
1
;
Un Chang YEO
;
Taeksun SONG
;
Ki Cheol KIM
;
Sung Yun PARK
;
Man Jung KIM
;
Sang Nae CHO
Author Information
1. Department of Microbiology and Institute for Immunology and Immunological Diseases, Brain Korea 21 Plus Project for the Medical Sciences, Yonsei University College of Medicine, Seoul 120-752, Korea. raycho@yuhs.ac
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
bovine tuberculosis;
IFN-gamma assay;
Mycobacterium bovis
- MeSH:
Animals;
Antigens, Bacterial/*diagnostic use;
Bacterial Proteins/diagnostic use;
Cattle;
Female;
Interferon-gamma Release Tests/*veterinary;
Mycobacterium bovis/*isolation & purification;
Polymerase Chain Reaction/veterinary;
Republic of Korea/epidemiology;
Tuberculosis, Bovine/*diagnosis/*epidemiology/microbiology
- From:Journal of Veterinary Science
2014;15(2):259-265
- CountryRepublic of Korea
- Language:English
-
Abstract:
The interferon-gamma (IFN-gamma) assay is employed as a complementary diagnostic test for bovine tuberculosis (BTB) in many countries. To simplify this assay, we established a 96-well plate format using the ESAT-6 and CFP-10 antigens and then employed it to determine the extent of Mycobacterium (M.) bovis infection in dairy herds with a history of BTB outbreaks in a country where only selective culling is practiced. The sensitivity and specificity of this IFN-gamma assay were 85.9% and 100%, respectively, based on comparison with the conventional single intradermal tuberculin test (SIDT). The IFN-gamma assay was also positive in 30.4% and 36.8% of SIDT-negative animals from herds with recent and remote BTB outbreaks, respectively. Of 14 SIDT-negative, IFN-gamma positive cattle, five (35.7%) were culture positive and an additional six were positive based on a polymerase chain reaction-based test for M. bovis. Therefore, the IFN-gamma assay has the potential to serve as a specific and sensitive test for M. bovis infection in dairy cattle. Further, the results indicated that a substantial portion of SIDT-negative animals in herds with previous BTB outbreaks were actually infected with M. bovis. Accordingly, the present selective-culling strategy may require modifications to include this more sensitive assay.
