Effects of ppk1 deletion on the drug susceptibility of uropathogenic Escherichia coli producing ESBLs.
10.3760/cma.j.cn112150-20220906-00876
- Author:
Jing Yi OU
1
;
Wan Shan CHEN
1
;
Mei Jun CHEN
1
;
Ling Zhai ZHAO
1
;
Ling Hua LI
2
;
Liang PENG
3
;
Lan LIANG
4
;
Ya Ling SHI
1
Author Information
1. Department of Clinical Laboratory, Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou 510440 China.
2. Infectious Department, Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou 510440, China.
3. Department of Clinical Laboratory, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou 510700, China.
4. The KingMed College of Laboratory Medicine,Guangzhou Medical University, Guangzhou 511436, China.
- Publication Type:Journal Article
- MeSH:
Humans;
beta-Lactamases/metabolism*;
Uropathogenic Escherichia coli/metabolism*;
Urinary Tract Infections;
Plasmids;
Membrane Proteins/genetics*;
Escherichia coli Infections;
Microbial Sensitivity Tests;
Anti-Bacterial Agents/pharmacology*
- From:
Chinese Journal of Preventive Medicine
2023;57(8):1238-1245
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect and the mechanism of ppk1 gene deletion on the drug susceptibility of uropathogenic Escherichia coli producing extended-spectrum beta-lactamases (ESBLs-UPEC). The study was an experimental study. From March to April 2021, a strain of ESBLs-UPEC (genotype was TEM combined with CTX-M-14) named as UE210113, was isolated from urine sample of the patient with urinary tract infection in the Laboratory Department of Guangzhou Eighth People's Hospital, meanwhile its ppk1 gene knock-out strain Δpk1 and complemented strain Δpk1-C were constructed by suicide plasmid homologous recombination technique, which was used to study the effect of ppk1 gene on ESBLs-UPEC drug sensitivity and its mechanism. The drug susceptibility of UE210113, Δpk1, and Δpk1-C were measured by Vitek2 Compact System and broth microdilution method. The quantitative expression of ESBLs, outer membrane protein and multidrug efflux systems encoding genes of UE210113, Δpk1 and Δpk1-C were performed by using qRT-PCR analysis. By using two independent sample Mann-Whitney U test, the drug susceptibility results showed that, compared with UE210113 strain, the sensitivities of Δpk1 to ceftazidime, cefepime, tobramycin, minocycline and cotrimoxazole were enhanced (Z=-2.121,P<0.05;Z=-2.236,P<0.05;Z=-2.236,P<0.05;Z=-2.121,P<0.05), and the drug susceptibility of Δpk1-C restored to the same as which of UE210113 (Z=0,P>0.05). The expression levels of ESBLs-enconding genes blaTEM and blaCTX-M-14 in Δpk1 were significantly down-regulated compared with UE210113, but the expression was not restored in Δpk1-C. The expression of outer membrane protein gene omp F in Δpk1 was significantly up-regulated, while the expression of omp A and omp C were down-regulated. The results showed that the expression of multidrug efflux systems encoding genes tol C, mdt A and mdtG were down-regulated in Δpk1 compared with UE210113. The expression of all of the outer membrane protein genes and the multidrug efflux systems genes were restored in Δpk1-C. In conclusion,the lost of ppk1 gene can affect the expression of the outer membrane protein and multidrug efflux systems encoding genes of ESBLs-UPEC, which increase the sensitivity of ESBLs-UPEC to various drugs.
