Screening of adjuvant for PD-L1 vaccine based on nitrated T cell epitope

VernacularTitle:基于硝基化T细胞表位的PD-L1疫苗佐剂的筛选
Author: Hongyou ZHOU ¹ ; Xiangdong GAO ; Wenbing YAO ; Hong TIAN
Author Information

1. 中国药科大学生命科学与技术学院江苏省生物药物成药性研究重点实验室,南京 211198
Keywords: tumor vaccine; adjuvant; Toll-like receptor; Poly(I:C)
From: Journal of China Pharmaceutical University 2024;55(3):397-403
CountryChina
Language:Chinese
Abstract: To enhance the anti-tumor activity of tumor vaccine targeting PD-L1 based on the nitrated T-epitope(PD-L1-NitraTh),this research compared several adjuvants with different mechanisms to screen out the adjuvant most suitable for PD-L1-NitraTh.The results showed that Poly(I:C),CPG1018,swollen knotted polysaccharide SGP2 and GM-CSF could enhance the immunogenicity of PD-L1-NitraTh when used as adjuvants,with the Poly(I:C)group inducing the highest antibody titer.The results of qPCR for T cell differentiation-related cytokines showed that Poly(I:C)reduced the expression of GATA3 and FoxP3,indicating a strong effect on CD4+T cell differentiation.Besides,compared with other adjuvants,Poly(I:C)could assist PD-L1-NitraTh to increase the infiltration of T cells as well as CD11b+cells within tumor,suggesting that Poly(I:C)may be the suitable adjuvant for tumor vaccines based on the nitrated T epitopes.