Molecular Genetic Study for FMR-1 Gene in Autistic Children.
- Author:
Kyung Mi KANG
1
;
Dong Il KWAK
;
Min Soo LEE
Author Information
1. Seoul National Mental Hospital, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Autistic children;
FMR-1 gene;
Fragile X syndrome
- MeSH:
Autistic Disorder;
Blotting, Southern;
Child*;
Diagnostic and Statistical Manual of Mental Disorders;
DNA;
Female;
Fragile X Syndrome;
Humans;
Male;
Molecular Biology*
- From:Journal of Korean Neuropsychiatric Association
1999;38(6):1479-1487
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: To elucidate an association of the fragile X syndrome with autism, Southern blot analysis was performed in 66 autistic children aged from 2 years to 11 years. METHODS: Subjects were 66 autistic children with of autistic disorder diagnosed by DSM-IV criteria and Childhood Autism Rating Scale-Korean version. Genomic DNA was extracted from peripheral blood and DNA was used to detect a FMR (Fragile Mental Retardation)-1 gene. Xho/PstI probes and two restriction enzymes (EcoRI, EagI)were used for Southern blot analysis. RESULTS: There were one boy with a methylated mosaic pattern and 3 boys and 2 girls with an unmethylated premutation band. But there was no full mutation pattern. CONCLUSION: Although the possibility of the relationship between autistic disorder and FMR-1 gene has been suggested, the results from this study do not provide any definite association of FMR-1 gene with autism in autistic children.
