Urinary MicroRNAs of Prostate Cancer: Virus-Encoded hsv1-miRH18 and hsv2-miR-H9-5p Could Be Valuable Diagnostic Markers.
- Author:
Seok Joong YUN
1
;
Pildu JEONG
;
Ho Won KANG
;
Ye Hwan KIM
;
Eun Ah KIM
;
Chunri YAN
;
Young Ki CHOI
;
Dongho KIM
;
Jung Min KIM
;
Seon Kyu KIM
;
Seon Young KIM
;
Sang Tae KIM
;
Won Tae KIM
;
Ok Jun LEE
;
Gou Young KOH
;
Sung Kwon MOON
;
Isaac Yi KIM
;
Jayoung KIM
;
Yung Hyun CHOI
;
Wun Jae KIM
Author Information
- Publication Type:Original Article
- Keywords: Prostate Cancer; Urine; Herpes Simplex; MicroRNA
- MeSH: Biomarkers; Biopsy; Cohort Studies; Diagnosis; Early Diagnosis; Herpes Simplex; Humans; Microarray Analysis; MicroRNAs*; Passive Cutaneous Anaphylaxis; Prostate; Prostate-Specific Antigen; Prostatic Hyperplasia; Prostatic Neoplasms*; Sensitivity and Specificity; Simplexvirus; Urologic Diseases
- From:International Neurourology Journal 2015;19(2):74-84
- CountryRepublic of Korea
- Language:English
- Abstract: PURPOSE: MicroRNAs (miRNAs) in biological fluids are potential biomarkers for the diagnosis and assessment of urological diseases such as benign prostatic hyperplasia (BPH) and prostate cancer (PCa). The aim of the study was to identify and validate urinary cell-free miRNAs that can segregate patients with PCa from those with BPH. METHODS: In total, 1,052 urine, 150 serum, and 150 prostate tissue samples from patients with PCa or BPH were used in the study. A urine-based miRNA microarray analysis suggested the presence of differentially expressed urinary miRNAs in patients with PCa, and these were further validated in three independent PCa cohorts, using a quantitative reverse transcriptionpolymerase chain reaction analysis. RESULTS: The expression levels of hsa-miR-615-3p, hsv1-miR-H18, hsv2-miR-H9-5p, and hsa-miR-4316 were significantly higher in urine samples of patients with PCa than in those of BPH controls. In particular, herpes simplex virus (hsv)-derived hsv1-miR-H18 and hsv2-miR-H9-5p showed better diagnostic performance than did the serum prostate-specific antigen (PSA) test for patients in the PSA gray zone. Furthermore, a combination of urinary hsv2-miR-H9-5p with serum PSA showed high sensitivity and specificity, providing a potential clinical benefit by reducing unnecessary biopsies. CONCLUSIONS: Our findings showed that hsv-encoded hsv1-miR-H18 and hsv2-miR-H9-5p are significantly associated with PCa and can facilitate early diagnosis of PCa for patients within the serum PSA gray zone.
