Apoptotic Activity of Curcumin and EF-24 in HTB-41 Human Salivary Gland Epidermoid Carcinoma Cells.
10.11620/IJOB.2015.40.2.063
- Author:
Ji Won KIM
1
;
Seul Ah LEE
;
Dae San GO
;
Byung Sun PARK
;
Su Gwan KIM
;
Sun Kyoung YU
;
Ji Su OH
;
Chun Sung KIM
;
Jeongsun KIM
;
Jong Tae PARK
;
Do Kyung KIM
Author Information
1. Oral Biology Research Institute, Chosun University School of Dentistry, Gwangju 501-759, Republic of Korea. kdk@chosun.ac.kr
- Publication Type:Original Article
- Keywords:
EF-24;
curcumin;
cell death;
apoptosis;
salivary gland epidermoid carcinoma cells
- MeSH:
Apoptosis;
Carcinoma, Squamous Cell*;
Caspase 3;
Cell Death;
Cell Nucleus;
Cell Proliferation;
Curcuma;
Curcumin*;
Drug Therapy;
Humans;
Intestinal Absorption;
Rhizome;
Salivary Glands*
- From:International Journal of Oral Biology
2015;40(2):63-69
- CountryRepublic of Korea
- Language:English
-
Abstract:
Curcumin (diferuloylmethane), a constituent of turmeric powder derived from the rhizome of Curcuma longa, has been shown to inhibit the growth of various types of cancer cells by regulating cell proliferation and apoptosis. However, a need exists to design more effective analogs because of curcumin's poor intestinal absorption. EF-24 (diphenyl difluoroketone), the monoketone analog of curcumin, has shown good efficacy in anticancer screens. However, the effects of curcumin and EF-24 on salivary gland epidermoid carcinoma cells are not clearly established. The main goal of this study was to investigate the effects of curcumin and EF-24 on cell growth and induction of apoptosis in human salivary gland epidermoid carcinoma cells. Our studies showed that curcumin and EF-24 inhibited the growth of HTB-41 cells in a dose- and time-dependent manner, and the potency of EF-24 was > 34-fold that of curcumin. Treatment with curcumin or EF-24 resulted in nuclear condensation and fragmentation in HTB-41 cells, whereas the control HTB-41 cell nuclei retained their normal regular and oval shape. Curcumin and EF-24 promoted proteolytic cleavages of procaspase-3/-7/-9, resulting in an increase in the amount of cleaved caspase-3/-7/-9 in the HTB-41 cells. Caspase-3 and -7 activities were detected in viable HTB-41 cells treated with curcumin or EF-24. These results suggest that the curcumin and EF-24 inhibit cell proliferation and induce apoptosis in HTB-41 human salivary gland epidermoid carcinoma cells, and that they may have potential properties as an anti-cancer drug therapy.
