Antimicrobial Effect of Coptidis rhizome Extract against Mutans Streptococci and Periodontopathogens.
10.11620/IJOB.2015.40.2.079
- Author:
Soon Nang PARK
1
;
Yun Kyong LIM
;
Joong Ki KOOK
Author Information
1. Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju, Korea. jkkook@chosun.ac.kr
- Publication Type:Original Article
- Keywords:
antimicrobial effect;
Coptidis rhizome;
mutans streptococci;
periodontopathogens
- MeSH:
Aggregatibacter actinomycetemcomitans;
Bacteria;
Cell Survival;
Chromatography;
Dental Caries;
Humans;
KB Cells;
Methanol;
Oral Hygiene;
Periodontal Diseases;
Porphyromonas gingivalis;
Prevotella intermedia;
Rhizome*;
Silica Gel;
Streptococcus mutans;
Streptococcus sobrinus;
Treponema denticola
- From:International Journal of Oral Biology
2015;40(2):79-83
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The purpose of the study was to investigate the antimicrobial activity of the methanol extract of Coptidis rhizome against the type strains of cariogenic bacteria, Streptococcus mutans and Streptococcus sobrinus, and the periodontopathogens, Porphyromonas gingivalis, Prevotella intermedia, Treponema denticola and Aggregatibacter actinomycetemcomitans. The antimicrobial activities of the crude extract and the methanol extract fractions of Coptidis rhizome separated by silica gel chromatography were evaluated by determining the minimal bactericidal concentration (MBC) values, using the microdilution method. The cell viability test of the extracts of Coptidis rhizome on the KB cells was also studied by methyl thiazolyl tetrazolium (MTT) assay. Our results showed that the 11th fraction (F11) of the methanol extract had the greatest antimicrobial activity against the tested bacteria, with no associated cytotoxicity on the KB cells, upto a concentration of 50 microg/ml. These results suggest that the silica gel chromatography fraction F11 of the methanol extract of Coptidis rhizome, could be useful in the development of oral hygiene products as an antimicrobial agent for the prevention of dental caries and periodontal diseases.
