Effect of Silencing subolesin and Parasitology enolase impairs gene expression, engorgement and reproduction in Haemaphysalis longicornis (Acari: Ixodidae) ticks
- Author:
Md. Samiul HAQUE
1
;
Mohammad Saiful ISLAM
;
Myung-Jo YOU
Author Information
- Publication Type:Research Report
- From:Journal of Veterinary Science 2024;25(3):e43-
- CountryRepublic of Korea
- Language:EN
-
Abstract:
Objective:The aim of the current research was to analyze the role of subolesin and enolase in feeding and reproduction of H. longicornis by gene silencing.
Methods:In this study, we used RNA interference to silence salivary enolase and subolesin in H. longicornis. Unfed female ticks injected with double-stranded RNA targeting subolesin and enolase were attached and fed normally on the rabbit's ear. Real-time polymerase chain reaction was used to confirm the extent of knockdown.
Results:Ticks in the subolesin or enolase dsRNA groups showed knockdown rates of 80% and 60% respectively. Ticks in the combination dsRNA (subolesin and enolase) group showed an 80% knockdown. Knockdown of subolesin and enolase resulted in significant depletion in feeding, blood engorgement weight, attachment rate, and egg laying. Silencing of both resulted in a significant (p < 0.05) reduction in tick engorgement, egg laying, egg hatching (15%), and reproduction.
Conclusions:and Relevance: Our results suggest that subolesin and enolase are an exciting target for future tick control strategies.
