Effect of Latanoprostene Bunod on the Permeability of Trabecular Meshwork Cells
10.3341/jkos.2024.65.2.139
- Author:
Hyeong Seok PARK
1
;
Jae Woo KIM
Author Information
1. Department of Ophthalmology, Daegu Catholic University School of Medicine, Daegu, Korea
- Publication Type:Original Article
- From:Journal of the Korean Ophthalmological Society
2024;65(2):139-144
- CountryRepublic of Korea
- Language:KO
-
Abstract:
Purpose:To investigate the effects of latanoprostene bunod (LBN) on nitric oxide (NO) production and permeability in human trabecular meshwork cells (HTMC).
Methods:HTMC were treated with 50 and 100 µM LBN and latanoprost free acid (LAT) for 30 minutes. Additionally, 100 µM LBN was co-exposed to 0.5 mM L-NAME (N-Nitroarginine methyl ester). Cellular viability and NO production were measured using MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) and Griess assays. The permeability and resistance of the HTMC monolayer were evaluated by trans-endothelial electrical resistance (TEER) and carboxyfluorescein permeability.
Results:Exposure to 100 µM LBN led to increased NO production, whereas co-exposure to L-NAME reduced NO production. Treatment with 100 µM LBN decreased the TEER of the HTMC monolayer. LBN exposure heightened carboxyfluorescein permeability, but co-exposure to 100 µM LBN and L-NAME reduced permeability. LAT treatment did not affect NO production or permeability.
Conclusions:LBN increased the permeability of the HTMC monolayer and increased NO production. Therefore, LBN might increase trabecular outflow in addition to promoting uveoscleral outflow.
