Expressions of the pERK1/2 and the cFos Proteins at an Early Stage of Transient Global Ischemia-reperfusion Injury in the Hippocampus of Rats.
- Author:
Chang Seok KO
1
;
Young Hyun YUN
;
Hun Cheol AHN
;
Jeong Woo CHOI
;
Dae Han WI
;
Su Jin YU
;
Jae Hwang PARK
Author Information
1. Department of Emergency Medicine, Wonkwang University School of Medicine, Iksan, Korea. ysoojin@wmc.wonkwang.ac.kr
- Publication Type:Original Article
- Keywords:
Hippocampus;
pERK1/2;
pMEK1/2;
cFos;
Transient global ischemia
- MeSH:
Animals;
CA1 Region, Hippocampal;
Cell Death;
Dentate Gyrus;
Hippocampus*;
Ischemia;
Neurons;
Prosencephalon;
Rats*;
Rats, Sprague-Dawley;
Reperfusion Injury*
- From:Journal of the Korean Society of Emergency Medicine
2004;15(6):475-486
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: This study was to evaluate temporal changes in the expressions of the phosphorylated extracellular-regulated kinase1/2 (pERK1/2), the phosphorylated MAPK/ERK kinase1/2 (pMEK1/2) and the cFos proteins in the hippocampus of rats following transient global ischemia. METHODS: Transient global ischemia was induced in the forebrains of Sprague-Dawley rats by using a 4-vessel occlusion for 20 min under anesthetic condition. Hematoxyline-eosin staining showed typical microscopic findings that represented neuronal cell death in hippocampal CA1 regions 5 days after transient global ischemia. Four-vessel occlusion-reperfusion produced ischemic injury in major forebrain structures, such as the striatum, the cortex and the hippocampus, in the finding of triphenyltetrazolium chloride (TTC) staining. RESULTS: A high density of pERK1/2 immunoreactivity existed in the pyramidal-cell layers of the CA2-3 regions and in the granular-cell layers of the dentate gyrus 5 min after ischemia. Following ischemia, expression of the pMEK1/2 protein showed temporal changes similar to that of the pERK1/2 protein. A significant expression of the cFos protein was noted in the pyramidal-cell layers of the CA2-3 regions and in the granular-cell layers of the dentate gyrus 2 hours after global ischemia. CONCLUSION: Intracellular signaling cascades of the ERK or the cFos protein take part in early cellular events in the hippocampus of rats in response to ischemic insult.
