Comparative analysis of cellulose acetate hemoglobin electrophoresis and high performance liquid chromatography for quantitative determination of hemoglobin A2.
- Author:
Shafi Mohammad KHOSA
1
;
Muhammad USMAN
;
Moinuddin MOINUDDIN
;
Hassan Osman MEHMOOD
;
Khansa QAMAR
Author Information
- Publication Type:Original Article
- Keywords: Cellulose acetate hemoglobin electrophoresis; Hemoglobin A2; High performance liquid chromatography
- MeSH: Anemia, Iron-Deficiency; beta-Thalassemia; Cellulose*; Chromatography, High Pressure Liquid; Chromatography, Liquid*; Cost-Benefit Analysis; Electrophoresis*; Hemoglobin A2*; Humans; Pakistan; Sensitivity and Specificity
- From:Blood Research 2015;50(1):46-50
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: The present study is designed to evaluate the reliability and cost effectiveness of cellulose acetate Hb electrophoresis and high performance liquid chromatography (HPLC) in the determination of HbA2 levels. METHODS: The test population comprised 160 individuals divided into four groups: normal individuals, beta-thalassemia trait (BTT) patients, iron deficiency anemia (IDA) patients, and co-morbid patients (BTT with IDA). HbA2 levels determined using cellulose acetate Hb electrophoresis and HPLC were compared. RESULTS: HbA2 levels were found to be diagnostic for classical BTT using either method. In co-morbid cases, both techniques failed to diagnose all cases of BTT. The sensitivity, specificity, and Youden's index for detection of the co-morbid condition was 69% and 66% for HPLC and cellulose acetate Hb electrophoresis, respectively. CONCLUSION: This study revealed that semi-automated cellulose acetate Hb electrophoresis is more suitable for use in beta-thalassemia prevention programs in low-income countries like Pakistan. This technique is easily available, simple and cost effective.
