Photochemically Induced Cerebral Ischemia in a Mouse Model.
- Author:
Sung Ku PARK
1
;
Jung Kil LEE
;
Kyung Sub MOON
;
Sung Pil JOO
;
Jae Hyoo KIM
;
Soo Han KIM
Author Information
1. Department of Neurosurgery, Chonnam National University Hospital, Medical School, Gwangju, Korea. jklee0261@yahoo.com
- Publication Type:Original Article
- Keywords:
Rose bengal;
Cerebral ischemia;
Photothrombosis;
Mouse
- MeSH:
Animals;
Brain;
Brain Ischemia*;
Cerebral Infarction;
Humans;
Infarction;
Injections, Intraperitoneal;
Ischemia;
Lighting;
Male;
Mice*;
Microvessels;
Middle Cerebral Artery;
Motor Cortex;
Rabeprazole;
Rats;
Rose Bengal;
Skull;
Stroke
- From:Journal of Korean Neurosurgical Society
2006;40(3):180-185
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: Middle cerebral artery occlusion(MCAO) has widely been used to produce ischemic brain lesions. The lesions induced by MCAO tend to be variable in size because of the variance in the collateral blood supply found in the mouse brain. To establish a less invasive and reproducible focal ischemia model in mice, we modified the technique used for rat photothrombosis model. METHODS: Male C57BL/6 mice were subjected to focal cerebral ischemia by photothrombosis of cortical microvessels. Cerebral infarction was produced by intraperitoneal injection of Rose Bengal, a photosensitive dye and by focal illumination through the skull. Motor impairment was assessed by the accelerating rotarod and staircase tests. The brain was perfusion-fixed for histological determination of infarct volume four weeks after stroke. RESULTS: The lesion was located in the frontal and parietal cortex and the underlying white matter was partly affected. A relatively constant infarct volume was achieved one month after photothrombosis. The presence of the photothrombotic lesion was associated with severe impairment of the motor performance measured by the rotarod and staircase tests. CONCLUSION: Photothrombotic infarction in mice is highly reproducible in size and location. This procedure can provide a simple method to produce cerebral infarction in a unilateral motor cortex lesion. In addition, it can provide a suitable model for study of potential neuroprotective and therapeutic agents in human stroke.
