Membrane protein alterations associated with anticancer drug resistance in mouse lymphoblastic leukemia L1210 cells.
10.12701/yujm.1993.10.2.432
- Author:
Seong Yong KIM
;
Sung Kweon SON
;
Jae Ryong KIM
;
Jung Hye KIM
- Publication Type:In Vitro ; Original Article
- MeSH:
Animals;
Cell Line;
Doxorubicin;
Drug Resistance*;
Electrophoresis;
Humans;
Membrane Proteins*;
Membranes*;
Mice*;
Phenotype;
Precursor Cell Lymphoblastic Leukemia-Lymphoma*;
Vincristine;
Weights and Measures
- From:Yeungnam University Journal of Medicine
1993;10(2):432-444
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Multidrug resistance(MDR) phenotype is frequently observed in animal and human cancer cell lines selected for in vitro resistance to a single chemotherapeutic agent. It is characterized by the diminished j drug accumulation and is related to the drug efflux mechanism in resistant cells. In the present study, adriamycin resistant cells(L1210-AdR6 : 10-6M adriamycin, -AdR5: 10-5M) and vincristine resistant cells (L1210-VcR7: 10-7M vincristine, -VcR6: 10-6M) were produced from mouse lymphoblastic leukemia cell line L1210. Growth profiles of survived cells were observed for 5 days with MTT(thiazolyl blue) assay and resistance was compared with IWdrug concentration of 50% survival reduction in absorbance). Resisrant cells proliferated more slowly than sensitive cell. Doubling times were 29.7hr in L1210, 68.7hr in L1210-AdR5 and 58.2hr in -VcR6. MDRs expressed as resistance factor were as follows, L1210-AdR5 was 76.4 times for vincristine, L1210-VcR6 was 96.4 times for adriamycin. The cell membrane proteins with three different M.W. were recognized to be related resistance, 220, 158, and 88 kd in L1210-AdR5, 158, 140 and 88 kd in L1210-VcR6 by SDS-PAG electrophoresis. Cell surface membrane proteins were identified by radio-iodination and autoradiogram. their molecular! weights were 158, 72.8. and 42.4 Kd in L1210-VcR6.
