Optimization of a Protocol for Isolating Cell-free DNA From Cerebrospinal Fluid
- Author:
Ho Hyun SONG
1
;
Hyeran PARK
;
Doohwan CHO
;
Hae In BANG
;
Hyuk-Jin OH
;
Jieun KIM
Author Information
- Publication Type:Brief Communication
- From:Annals of Laboratory Medicine 2024;44(3):294-298
- CountryRepublic of Korea
- Language:English
- Abstract: A standardized protocol for the isolation of cell-free DNA (cfDNA) from cerebrospinal fluid (CSF) is lacking. Therefore, we established a cfDNA isolation protocol optimized for clinical CSF specimens, integrating acceptable modifications and using artificial CSF generated from remnant CSF spiked with reference cell-free tumor DNA (ctDNA). We compared the isolation yields of in vitro diagnostic (IVD)-certified column-based (CB) and magnetic beadbased (MB) isolation. Furthermore, we modified both methods, including pre- and postelution steps. To confirm ctDNA integrity and quantify the variant allele frequency after isolation, we performed droplet digital PCR (ddPCR) targeting IDH1 R132C in the reference ctDNA. MB isolation had a higher yield than CB isolation (P < 0.0001), and post-isolation vacuum increased the final concentration in both methods, with little effect on cfDNA integrity. Our study provides a protocol to maximize CSF-ctDNA concentrations in IVD testing and future studies.
