Simultaneous Determination of Five Compounds in Descurainia sophia by HPLC-DAD
- Author:
Wooseung YANG
1
;
Choong Je MA
Author Information
- From:Natural Product Sciences 2023;29(4):305-311
- CountryRepublic of Korea
- Language:English
- Abstract: Descurainia sophia was commonly used for treating of cough and asthma in Asian country such as Korea, China and Japan. According to our previous study, it also had anti-influenza activity and anti-influenza compounds were isolated and identified. In this study, simultaneous determination analytical method of isolated five compounds such as daucosterol (1), helveticoside (2), isorhamnetin (3), quercetin (4), and sinapic acid (5) was established by using HPLC-DAD. The HPLC analysis was carried out using a Dionex C 18 column (5 µm particle size, 120 Å pore size, 4.6 mm × 150 mm dimensions) at a constant temperature of 25 o C. The mobile phase consisted of a blend of 0.1% trifluoroacetic acid (TFA) and acetonitrile and delivered at a flow rate of 1 mL/min. Method validation was conducted to assess its reliability and accuracy. The calibration curve exhibited excellent linearity, with an R² value exceeding 0.9994, demonstrating the method’s capability to accurately quantify the compounds across a range of concentrations. Limit of Detection (LOD) ranged from 0.93 to 3.19 µg/ mL, and limit of quantification (LOQ) spanned from 2.79 to 9.57 µg/mL, respectively. The relative standard deviations (RSD) values of precision test, intra- and inter- day, were less than 1.28 % and 1.14%. The accuracy test results ranged from 100.81% to 105.31% and RSD values were less than 1.26 %. These results showed that the HPLC-DAD method was very reliable and accurate for the quantity analysis of eight compounds in D. sophia extract for quality control.
