Copper Tolerance of Novel Rhodotorula sp. Yeast Isolated from Gold Mining Ore in Gia Lai, Vietnam
10.1080/12298093.2023.2274648
- Author:
Kim Cuc Thi NGUYEN
1
;
Phuc Hung TRUONG
;
Cuong Tu HO
;
Cong Tuan LE
;
Khoa Dang TRAN
;
Tien Long NGUYEN
;
Manh Tuan NGUYEN
;
Phu Van NGUYEN
Author Information
1. Institute of Biotechnology, Hue University, Thua Thien Hue, Vietnam
- Publication Type:RESEARCH ARTICLE
- From:Mycobiology
2023;51(6):379-387
- CountryRepublic of Korea
- Language:English
-
Abstract:
In this study, twenty-five yeast strains were isolated from soil samples collected in the gold mining ore in Gia Lai, Vietnam. Among them, one isolate named GL1 T could highly tolerate Cu 2+ up to 10 mM, and the isolates could also grow in a wide range of pH (3–7), and tem perature (10–40 ℃). Dried biomass of GL1 was able to remove Cu 2+ effectively up to 90.49% with a maximal biosorption capacity of 18.1 mg/g at pH 6, temperature 30 ℃, and incuba tion time 60 min. Sequence analysis of rDNA indicated this strain was closely related to Rhodotorula mucilaginosa but with 1.53 and 3.46% nucleotide differences in the D1/D2 domain of the 28S rRNA gene and the ITS1-5.8S rRNA gene-ITS2 region sequence, respect ively. Based on phylogenetic tree analysis and the biochemical characteristics, the strain appears to be a novel Rhodotorula species, and the name Rhodotorula aurum sp. nov. is pro posed. This study provides us with more information about heavy metal-tolerant yeasts and it may produce a new tool for environmental control and metal recovery operations.
