Promising improvement in infected Wound Healing in Type two Diabetic rats by Combined effects of conditioned medium of human adipose‑derived stem cells plus Photobiomodulation
10.1186/s42826-023-00178-z
- Author:
Kaysan SOHRABI
1
;
Houssein AHMADI
;
Abdollah AMINI
;
Behnaz AHRABI
;
Atarodalsadat MOSTAFAVINIA
;
Hamidreza OMIDI
;
Mansooreh MIRZAEI
;
Fatemeh Fadaei FATHABADY
;
Mohammadjavad FRIDONI
;
Maryam RAHMANNIA
;
Sufan CHIEN
;
Mohammad BAYAT
Author Information
1. School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
- Publication Type:RESEARCH
- From:Laboratory Animal Research
2023;39(4):356-370
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background:We aimed to examine the accompanying and solo impacts of conditioned medium of human adiposederived stem cells (h-ASC-COM) and photobiomodulation (PBM) on the maturation stage of an ischemic infected delayed-healing wound model (IIDHWM) of rats with type 2 diabetes (TIIDM).
Results:Outcomes of the wound closure ratio (WCR) results, tensiometrical microbiological, and stereological assessment followed almost identical patterns. While the outcomes of h-ASC-COM + PBM, PBM only, and h-ASCCOM only regimes were significantly better for all evaluated methods than those of group 1(all, p < 0.001), PBM alone and h-ASC-COM + PBM therapy achieved superior results than h-ASC-COM only (ranged from p = 0.05 to p < 0.001). In terms of tensiometrical and stereological examinations, the results of h-ASC-COM + PBM experienced better results than the PBM only (all, p < 0.001).
Conclusions:h-ASC-COM + PBM, PBM, and h-ASC-COM cures expressively accelerated the maturation stage in the wound healing process of IIDHWM with MRSA in TIIDM rats by diminishing the inflammatory reaction, and the microbial flora of MRSA; and increasing wound strength, WCR, number of fibroblasts, and new blood vessels. While the h-ASC-COM + PBM and PBM were more suitable than the effect of h-ASC-COM, the results of h-ASCCOM + PBM were superior to PBM only.
