Inflammatory Factors Predicting Dry Eye Syndrome in a Model Using Osmotic Pressure
10.3341/jkos.2023.64.12.1191
- Author:
Keon Woo PARK
1
;
Jae Woong KOH
Author Information
1. Department of Ophthalmology, Chosun University College of Medicine, Gwangju, Korea
- Publication Type:Original Article
- From:Journal of the Korean Ophthalmological Society
2023;64(12):1191-1197
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Purpose:To identify inflammatory factors that may serve as biomarkers for dry eye syndrome using a hyperosmotic dry eye syndrome model.
Methods:Cultured human conjunctival epithelial cells were subjected to 400, 450, 500, and 550 mOsm/L NaCl, and cell viability was assessed in response to osmolarity. The relative expression of inflammatory factors was evaluated by real-time polymerase chain reaction of interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor (TNF)-α, metalloproteinase (MMP)-2, and MMP-9 according to the NaCl concentration.
Results:An increase in the concentration of NaCl led to a notable decrease in cell viability (p < 0.05). IL-8, TNF-α, and MMP-9 expression levels increased significantly in proportion to NaCl concentration (p < 0.05).
Conclusions:IL-8, TNF-α, and MMP-9 may serve as effective biomarkers in dry eye studies.
