Phylogenetic Groups and Virulence Factors of Escherichia coli Causing Urinary Tract Infection in Children.
10.14776/piv.2015.22.3.194
- Author:
Ji Mok KIM
1
;
Eun Young CHO
;
Jae Ho LEE
Author Information
1. Department of Pediatrics, Chungnam National University School of Medicine, Daejeon, Korea. immlee@cnu.ac.kr
- Publication Type:Original Article
- Keywords:
Escherichia coli;
Virulence factor;
Phylogenetic groups;
Acute pyelonephritis
- MeSH:
Bacterial Infections;
Child*;
Cystitis;
Diagnosis;
Escherichia coli*;
Escherichia*;
Humans;
Korea;
Polymerase Chain Reaction;
Pyelonephritis;
Urinary Tract Infections*;
Urinary Tract*;
Vesico-Ureteral Reflux;
Virulence Factors*;
Virulence*
- From:Pediatric Infection & Vaccine
2015;22(3):194-200
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Urinary tract infection (UTI) is a common bacterial infection in children and Escherichia coli is a predominant pathogen. The purpose of this study is to evaluate phylogenetic groups and virulence factors of E. coli causing UTI in children in Korea. METHODS: From October 2010 to April 2013, urinary E. coli strains were isolated from the 33 pediatric patients of UTI. Multiplex polymerase chain reactions were performed to evaluate the phylogenetic groups and 5 virulence factor genes (fimH, sfa, papA, hylA, and cnf1) of E. coli. Distribution of molecular characteristics of E. coli was analyzed by clinical diagnosis and accompanying vesicoureteral reflux (VUR). RESULTS: Most (84.8%) uropathogenic E. coli were belonged to phylogenetics group B2 and the others (15.2%) were belonged to group D. The virulence factors were distributed as: fimH (100%), sfa (100%), hylA (63.6%), cnfI (63.6%), and papA (36.4%). According to clinical diagnosis, phylogenetic distribution of E. coli strain was 92.3% of B2 and 7.7% of D in acute pyelonephritis and 57.1% of B2 and 42.9% of D in cystitis. Distribution of virulence factors was similar in both groups. In patients with acute pyelonephritis, phylogenetic distribution was similar in VUR and non-VUR group, but proportion of papA genes were lower in VUR group than that of non-VUR group (43.8% vs. 20.0%, P=0.399). CONCLUSIONS: This study provides current epidemiologic molecular data of E. coli causing pediatric UTI in Korea and will be a fundamental for understanding the pathogenesis of pediatric UTI.
