Tissue Engineering of the Intervertebral Disc with Cultured Nucleus Pulposus Cells Using Atelocollagen Scaffold and Gene Therapy.
10.4184/jkss.2010.17.2.49
- Author:
Hak Sun KIM
1
;
Kwang Il LEE
;
Hyang KIM
;
Un Hye KWON
;
Mi Ran NAM
;
Ju Woong JANG
;
In Je CHO
;
Boram KIM
;
Hwan Mo LEE
;
Seong Hwan MOON
Author Information
1. Department of Orthopaedic Surgery, Yonsei University College of Medicine, Seoul, Korea. shmoon@yuhs.ac
- Publication Type:Original Article
- Keywords:
Intervertebral disc;
Collagen;
Scaffold;
TGF-beta1;
BMP-2
- MeSH:
Aggrecans;
Collagen;
Collagen Type I;
Collagen Type II;
DNA;
Genetic Therapy;
Intercellular Signaling Peptides and Proteins;
Intervertebral Disc;
Osteocalcin;
Proteoglycans;
Regeneration;
RNA, Messenger;
Tissue Engineering;
Tissue Therapy;
Transforming Growth Factor beta1
- From:Journal of Korean Society of Spine Surgery
2010;17(2):49-56
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
STUDY DESIGN: This is an in-vitro experiment using rabbit intervertebral disc (IVD) cells and growth factors. OBJECTIVES: We wanted to determine the effect of types I, and II atelocollagen and growth factor gene therapy for matrix regeneration of rabbit IVD cells. SUMMARY OF THE LITERATURE REVIEW: Adenovirus-medicated growth factor gene therapy is efficient for matrix regeneration of the IVD. Atellocollagen has provided a favorable environment for matrix synthesis. However, a combined approach using gene and cell therapy in an atelocollagen scaffold has not yet been attempted. MATERIALS AND METHODS: Rabbit IVD cells were transduced with Ad/TGF-beta1 and Ad/BMP-2. The cells were then implanted to the atelocollagen scaffold. The [methyl-3H]thymidine incorporation for DNA synthesis and the [35S]sulfur incorporation for proteoglycan synthesis were measured. RT-PCR was performed for assessing the aggrecan, collagen type I, collagen type II and osteocalcin mRNA expressions. RESULTS: The rabbit IVD cells with Ad/TGF-beta1 and that were cultured in type I atelocollagen showed a 130% increase in new proteoglycan synthesis, while the rabbit IVD cells with Ad/TGF-beta1 and that were cultured in type II atelocollagen showed a 180% increase in new proteoglycan synthesis (p<0.05). The rabbit IVD cells with Ad/BMP-2 and that were cultured in type I atelocollagen showed a 70% increase in new proteoglycan synthesis, while the rabbit IVD cells with Ad/BMP-2 and that were cultured in type II atelocollagen showed a 95% increase (p<0.05). Rabbit IVD cells with Ad/TGF-beta1 and Ad/BMP-2 and that were cultured in type I and II atelocollagen demonstrated increased collagen type I and II mRNA expressions without an osteocalcin mRNA expression (p<0.05). CONCLUSION: Cell and gene therapy in an atelocollagen scaffold provided a efficient mechanism for chondrogenic matrix regeneration of rabbit IVD cells.
