Effect of Baicalein on Osteogenic Differentiation of MC3T3-E1 Cells and Its Bacteriostasis against Common Oral Bacteria
10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).20240617.009
- VernacularTitle:黄芩素对MC3T3-E1体外成骨分化及对口腔常见菌的抑菌作用
- Author:
Yue WANG
1
;
Fangchen LIN
;
Qi SU
;
Xia HUANG
;
Zhiying ZHOU
Author Information
1. 暨南大学口腔医学院,广东 广州 510632
- Keywords:
preosteoblasts;
baicalein;
osteogenesis;
bacteriostasis;
differentiation;
proliferation
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2024;45(4):602-612
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the impact of varying concentrations of baicalein on the proliferation and biological responses of MC3T3-E1 cells,as well as the antibacterial efficacy of baicalein against prevalent oral bacteria,and to elucidate the underlying mechanisms.[Methods]MC3T3-E1 cells were exposed to different concentrations of baicalein(0,6,12,18,and 24 μmol/L)and cell viability was determined by using the CCK-8 assay.Alkaline phosphatase(ALP)activity of MC3T3-E1 cells following osteogenic induction was assessed.RT-PCR was used to examine the expression of RunX2,BMP2,and Osterix.After 24 hours of treatment,the antibacterial potential of baicalein against Escherichia coli,Staphylococcus Aureus and Streptococcus Sanguis was evaluated by using the K-B paper disk method.[Results]Baicalein exhibited a modest reduction in proliferation of MC3T3-E1 cells but without affecting their sustained proliferation.Baicalein at a concentration of 18 μmol/L enhanced ALP activity of MC3T3-E1 cells,upregulated BMP2 and Osterix expression,downregulated RunX2 expression,significantly inhibited the proliferation of Staphylococcus Aureus and Streptococcus Sanguis(P<0.05).[Conclusions]Baicalein at an optimal concentration(18 μmol/L)demonstrated a promotional effect on the osteogenic differentiation of MC3T3-E1 cells and effectively suppressed the proliferation of common oral bacteria,including Staphylococcus Aureus and Streptococcus Sanguis.
