Effect of Baicalein on Osteogenic Differentiation of MC3T3-E1 Cells and Its Bacteriostasis against Common Oral Bacteria
10.13471/j.cnki.j.sun.yat-sen.univ（med.sci）.20240617.009
- VernacularTitle:黄芩素对MC3T3-E1体外成骨分化及对口腔常见菌的抑菌作用
- Author:
Yue WANG
1
;
Fangchen LIN
1
;
Qi SU
1
;
Xia HUANG
1
;
Zhiying ZHOU
2
Author Information
1. College of Stomatology， Jinan University， Guangzhou 510632， China
2. Department of Orthodontics， The First Affiliated Hospital of Jinan University， Guangzhou 510630， China
- Publication Type:Journal Article
- Keywords:
preosteoblasts;
baicalein;
osteogenesis;
bacteriostasis;
differentiation;
proliferation
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2024;45(4):602-612
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the impact of varying concentrations of baicalein on the proliferation and biological responses of MC3T3-E1 cells， as well as the antibacterial efficacy of baicalein against prevalent oral bacteria， and to elucidate the underlying mechanisms. MethodsMC3T3-E1 cells were exposed to different concentrations of baicalein （0， 6， 12， 18， and 24 μmol/L） and cell viability was determined by using the CCK-8 assay. Alkaline phosphatase （ALP） activity of MC3T3-E1 cells following osteogenic induction was assessed. RT-PCR was used to examine the expression of RunX2， BMP2， and Osterix. After 24 hours of treatment， the antibacterial potential of baicalein against Escherichia coli， Staphylococcus Aureus and Streptococcus Sanguis was evaluated by using the K-B paper disk method. ResultsBaicalein exhibited a modest reduction in proliferation of MC3T3-E1 cells but without affecting their sustained proliferation. Baicalein at a concentration of 18 μmol/L enhanced ALP activity of MC3T3-E1 cells， upregulated BMP2 and Osterix expression， downregulated RunX2 expression， significantly inhibited the proliferation of Staphylococcus Aureus and Streptococcus Sanguis （P < 0.05）. ConclusionsBaicalein at an optimal concentration （18 μmol/L） demonstrated a promotional effect on the osteogenic differentiation of MC3T3-E1 cells and effectively suppressed the proliferation of common oral bacteria， including Staphylococcus Aureus and Streptococcus Sanguis.
