Shuxuetong Inhibits Bim-dependent Apoptosis of Cerebellar Granule Neurons
10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).20240711.003
- VernacularTitle:疏血通抑制Bim依赖的小脑颗粒神经元凋亡
- Author:
Shenhao PAN
1
;
Dongfang CAO
;
Fanyi ZHAO
;
Sijie ZHAO
;
Chenghao ZHANG
;
Jianfeng LIANG
;
Jianwei WU
;
Zhongmin YUAN
Author Information
1. 广州医科大学第二临床学院,广东 广州 510260
- Keywords:
cerebellar granule neurons;
apoptosis;
shuxuetong;
hirudin;
Bim;
VEGF
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2024;45(4):549-556
- CountryChina
- Language:Chinese
-
Abstract:
[Objective]To investigate the effect and mechanism of Shuxuetong and its main component hirudin on the apoptosis of cerebellar granule neurons(CGNs)in Sprague-Dawley(SD)rats.[Methods]CGNs incubated in vitro for 7 days were divided into survival control group or 25 K group(cultured in medium containing 25 mmol/L KCL)and apopto-sis group or 5 K group(cultured in medium containing 5 mmol/L KCL).CGNs were separately treated with proportionally diluted and different concentrations of Shuxuetong(1/50,1/40,1/30,1/20 and 1/10)and the corresponding different con-centrations of hirudin(2,2.5,3.34,5 and 10 U/mL).Hoechst staining was performed to analyze the apoptosis.Western blot was used to detect the expression levels of Cleaved Caspase-3,Bim and VEGF.[Results]Hoechst staining showed that 5 K group had a higher apoptosis rate than 25 K group.In 25 K group,there was no significant change in the apoptosis rate between neurons treated with different concentrations of Shuxuetong and hirudin,but significant changes was found in 5 K group and the higher the concentration,the lower the apoptosis rate.Western blot results revealed that,compared with control neurons in 5 K group,Shuxuetong injection and hirudin treatments resulted in a decrease of Cleaved Caspase-3 and Bim expression,but an increase of VEGF protein.[Conclusions]Shuxuetong and its main component hirudin inhibits the apoptosis of CGNs through suppressing proapoptotic BH3-only protein Bim.
