The molecular mechanism of the Tianma preparation in the treating migraine by adenosine pathway
- VernacularTitle:天麻制剂通过腺苷途径治疗偏头痛相关的分子机制研究
- Author:
Haifei ZHENG
1
;
Jinbo CHEN
1
;
Weiwei SONG
1
Author Information
- Publication Type:Journal Article
- Keywords: Migraine; Electrical stimulation of the trigeminal ganglion; Gastrodin; Vanillin; 4-hydroxybenzyl alcohol; β-sitosterol; CGRP; Adenosine A1 receptor
- From: Journal of Apoplexy and Nervous Diseases 2020;37(3):255-260
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the effect of the active ingredients of Tianma preparation on the expression of calcitonin gene-related peptide（CGRP） and adenosine A1 receptor in migraine model rats. Methods Eighty-four SPF rats were randomly divided into 7 groups （n=12）：sham operation group （group A），electrical stimulation of the trigeminal ganglion group（ESTG group），Sumatriptan group （group C），gastrodin group （group D），4-hydroxybenzyl alcohol group （group E），vanilanol group （group F），β-sitosterol group （group G）. The enzyme-linked immuno sorbent assay （ELISA），immunofluorescence and western-blot techniques were used to dected the effect of the active components of Tianma preparation on the expression of CGRP and adenosine A1 receptor. Results Compared with group A，the expression of CGRP in trigeminal ganglia （TG） and trigeminal nucleus caudalis （TNC） in group B rats was significantly increased，while the expression of adenosine A1 receptor was significantly decreased，with statistically significant differences （P<0.01）. Compared with group B，the expression of CGRP in TG and TNC in group C and D was significantly decreased，and the expression of adenosine A1 receptor was significantly increased，with statistically significant differences （P<0.01）. There was no significant difference between group E，F，G and group B （P>0.05）. Compared with group C，the expression of CGRP and adenosine A1 receptor in TG and TNC of rats in group D was not statistically significant （P>0.05）. Conclusion Like sumatriptan，The preventive application of gastrodin can alleviate the migraine attack，while the effects of vanillin，4-hydroxybenzyl alcoholand β-sitosterol were not good enough. In addition，gastrodin can inhibit the occurrence of migraine by activating adenosine A1 receptor and inhibiting the release of CGRP.
- Full text:2024080212252906339The molecular mechanism of the Tianma preparation in the treating migraine by adenosine pathway.pdf
